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Screening for autoa...
Screening for autoantibody targets in post-vaccination narcolepsy using proteome arrays
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- Lind, Alexander (author)
- Lund University,Lunds universitet,Pediatrisk endokrinologi,Forskargrupper vid Lunds universitet,Paediatric Endocrinology,Lund University Research Groups,Skåne University Hospital,Lund Univ, Skane Univ Hosp SUS, CRC, Dept Clin Sci Malmö, Jan Waldenstroms Gata 35, S-21428 Malmö, Sweden.
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- Eriksson, Daniel (author)
- Karolinska Institutet,Karolinska Inst, Ctr Mol Med, Dept Med Solna, Stockholm, Sweden.;Karolinska Univ Hosp, Dept Endocrinol Metab & Diabet, Stockholm, Sweden.
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- Akel, Omar (author)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Department of Clinical Sciences, Malmö,Faculty of Medicine,Skåne University Hospital,Lund Univ, Skane Univ Hosp SUS, CRC, Dept Clin Sci Malmö, Jan Waldenstroms Gata 35, S-21428 Malmö, Sweden.
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- Ramelius, Anita (author)
- Lund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups,Skåne University Hospital,Lund Univ, Skane Univ Hosp SUS, CRC, Dept Clin Sci Malmö, Jan Waldenstroms Gata 35, S-21428 Malmö, Sweden.
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- Palm, Lars (author)
- Skane Univ Hosp SUS, Dept Paediat, Sect Paediat Neurol, Malmö, Sweden.,Skåne University Hospital
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- Lernmark, Åke (author)
- Lund University,Lunds universitet,Celiaki och diabetes,Forskargrupper vid Lunds universitet,Celiac Disease and Diabetes Unit,Lund University Research Groups,Skåne University Hospital,Lund Univ, Skane Univ Hosp SUS, CRC, Dept Clin Sci Malmö, Jan Waldenstroms Gata 35, S-21428 Malmö, Sweden.
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- Kämpe, Olle, 1956- (author)
- Uppsala University,Karolinska Institutet,Uppsala universitet,Institutionen för medicinska vetenskaper,Science for Life Laboratory, SciLifeLab,Karolinska Inst, Ctr Mol Med, Dept Med Solna, Stockholm, Sweden.;Karolinska Univ Hosp, Dept Endocrinol Metab & Diabet, Stockholm, Sweden.
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- Elding Larsson, Helena (author)
- Lund University,Lunds universitet,Pediatrisk endokrinologi,Forskargrupper vid Lunds universitet,Paediatric Endocrinology,Lund University Research Groups,Skåne University Hospital,Lund Univ, Skane Univ Hosp SUS, CRC, Dept Clin Sci Malmö, Jan Waldenstroms Gata 35, S-21428 Malmö, Sweden.
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- Landegren, Nils (author)
- Karolinska Institutet
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(creator_code:org_t)
- Wiley, 2020
- 2020
- English.
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In: Scandinavian Journal of Immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 91:4
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Abstract
Subject headings
Close
- Narcolepsy type 1 (NT1) is a chronic sleep disorder caused by a specific loss of hypocretin‐producing neurons. The incidence of NT1 increased in Sweden, Finland and Norway following Pandemrix®‐vaccination, initiated to prevent the 2009 influenza pandemic. The pathogenesis of NT1 is poorly understood, and causal links to vaccination are yet to be clarified. The strong association with Human leukocyte antigen (HLA) DQB1*06:02 suggests an autoimmune pathogenesis, but proposed autoantigens remain controversial. We used a two‐step approach to identify autoantigens in patients that acquired NT1 after Pandemrix®‐vaccination. Using arrays of more than 9000 full‐length human proteins, we screened the sera of 10 patients and 24 healthy subjects for autoantibodies. Identified candidate antigens were expressed in vitro to enable validation studies with radiobinding assays (RBA). The validation cohort included NT1 patients (n = 39), their first‐degree relatives (FDR) (n = 66), population controls (n = 188), and disease controls representing multiple sclerosis (n = 100) and FDR to type 1 diabetes patients (n = 41). Reactivity towards previously suggested NT1 autoantigen candidates including Tribbles homolog 2, Prostaglandin D2 receptor, Hypocretin receptor 2 and α‐MSH/proopiomelanocortin was not replicated in the protein array screen. By comparing case to control signals, three novel candidate autoantigens were identified in the protein array screen; LOC401464, PARP3 and FAM63B. However, the RBA did not confirm elevated reactivity towards either of these proteins. In summary, three putative autoantigens in NT1 were identified by protein array screening. Autoantibodies against these candidates could not be verified with independent methods. Further studies are warranted to identify hypothetical autoantigens related to the pathogenesis of Pandemrix®‐induced NT1.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- antigens
- peptides
- epitopes
- autoantibodies
- autoimmunity
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Lind, Alexander
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Eriksson, Daniel
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Akel, Omar
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Ramelius, Anita
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Palm, Lars
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Lernmark, Åke
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show more...
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Kämpe, Olle, 195 ...
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Elding Larsson, ...
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Landegren, Nils
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Scandinavian Jou ...
- By the university
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Uppsala University
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Lund University
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Karolinska Institutet