Affinity apheresis for treatment of bacteremia caused by Staphylococcus aureus and/or methicillin-resistant S. aureus (MRSA).

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Affinity apheresis for treatment of bacteremia caused by Staphylococcus aureus and/or methicillin-resistant S. aureus (MRSA).

Mattsby-Baltzer, Inger, 1949 (författare): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine

Bergström, Tomas, 1950 (författare): Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine

McCrea, Keith (författare)

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Ward, Robert (författare)

Adolfsson, Lars (författare)

Larm, Olle (författare)

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(creator_code:org_t)

2011
2011
Engelska.
Ingår i: Journal of microbiology and biotechnology. - 1738-8872. ; 21:6, s. 659-64

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Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

Stäng

Staphylococcus aureus (SA) bacteremia is associated with high mortality, and often results in metastatic infections. The methicillin-resistant SA (MRSA) is an urgent health care issue, as nosocomial infections with these bacteria represent limited treatment alternatives. Samples of whole blood containing challenge inoculums of SA and MRSA strains were passed through columns packed with surface-heparinized polyethylene beads. The bound bacteria were eluted and quantitatively determined by culturing and by real-time PCR. Significant amounts of both SA and MRSA adhered to the heparinized beads (more than 65% of inoculated bacteria). After rinsing with buffer at high ionic strength, viable bacteria or bacterial DNA were eluted from the columns, indicating that the binding was specific. The conclusions that can be made from these experiments are that, as earlier reported in the literature, the high affinity of SA to heparin is retained in whole blood, and MRSA in whole blood binds to heparin with similar or higher affinity than SA. It should be possible to lower the amount of SA and/or MRSA from the blood of infected patients to levels that could be taken care of by the immune system. In previous studies, we have shown that passing blood from septic patients over beads coated with end-point-attached, biologically active heparin is a useful technique for regulating the levels of heparin-binding cytokine. These findings in combination with the present findings indicate the possibility of creating an apheresis technology for treatment of sepsis caused by SA and/or MRSA.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)

Nyckelord

Bacteremia
microbiology
therapy
Bacterial Adhesion
Biotechnology
methods
Blood Component Removal
methods
Colony Count
Microbial
Cross Infection
microbiology
therapy
Heparin
metabolism
Humans
Immobilization
Methicillin-Resistant Staphylococcus aureus
genetics
isolation & purification
metabolism
Microspheres
Polyethylene
chemistry
Polymerase Chain Reaction
methods
Staphylococcal Infections
microbiology
therapy
Staphylococcus aureus
genetics
isolation & purification
metabolism

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Av författaren/redakt...: Mattsby-Baltzer, ...; Bergström, Tomas ...; McCrea, Keith; Ward, Robert; Adolfsson, Lars; Larm, Olle

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Av lärosätet: Göteborgs universitet

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