Localised lipid accumulation detected in infarcted mouse heart tissue using ToF-SIMS

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Sämfors, Sanna,1987Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (författare)

Localised lipid accumulation detected in infarcted mouse heart tissue using ToF-SIMS

Artikel/kapitelEngelska2019

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Elsevier BV,2019

Nummerbeteckningar

LIBRIS-ID:oai:gup.ub.gu.se/278147
https://gup.ub.gu.se/publication/278147URI
https://doi.org/10.1016/j.ijms.2017.09.012DOI
https://research.chalmers.se/publication/501622URI
https://research.chalmers.se/publication/508446URI

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Språk:engelska

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Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Cardiovascular disease (CVD) is largely related to complications from atherosclerotic disease such as myocardial infarction (MI) and ischemic stroke and accounts for more than 30% of overall global mortality. Understanding the biochemical changes that occur in cardiac tissue following myocardial infarction is critical for clarifying the mechanisms underlying the impaired heart function seen after a myocardial infarction. Lipids have been shown to accumulate in ischemic cardiac tissue following an infarction. Recent data indicate that this cardiac lipid accumulation induces apoptosis and loss of muscle cells during the post-infarction period, which aggravate the functional impairment in the heart and limit its adaptive capacity for compensatory remodelling. It is therefore important to identify the lipids and molecular mechanisms that induce these destructive responses. In this study, the spatial distribution of lipids in mouse cardiac tissue after surgically induced infarction were identified using ToF-SIMS imaging with a gas cluster ion beam (GCIB). The benefits of frozen hydrated analysis versus freeze dried sample preparation were assessed as was the suitability of different multivariate analysis techniques for identification of localised chemical changes in the tissue. Results show that differences in intensity of the peaks in the mass spectrum corresponding to different lipids can be detected between the infarcted region of the heart and normal tissue region as well as specific accumulation of acyl-carnitine species at the boundary of the damaged region. Different spatial distributions of lipids were detected in both positive and negative ion mode providing insights into the changes in lipid metabolism following infarction. The ToF-SIMS results were compared with conventional lipidomics where although many lipid classes show similar changes between infarcted and non-infarcted hearts the ToF-SIMS data revealed differences due to salt adduct formation and most importantly where the changes in lipid signal are highly localised at the border between the infarcted and non-infarcted regions of the heart. (C) 2017 Elsevier B.V. All rights reserved.

Ämnesord och genrebeteckningar

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
NATURVETENSKAP Fysik hsv//swe
NATURAL SCIENCES Physical Sciences hsv//eng
Myocardial infarction
Lipid accumulation
ToF-SIMS
Imaging
Gas cluster ion beams
Mouse heart
ion mass-spectrometry
electrospray-ionization
identification
beams
quantification
phospholipids
lipotoxicity
cardiolipin
metabolism
separation
Physics
Spectroscopy
ToF-SIMS

Biuppslag (personer, institutioner, konferenser, titlar ...)

Ståhlman, Marcus,1975Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,University of Gothenburg(Swepub:gu)xstahm (författare)
Klevstig, MartinaGothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,University of Gothenburg(Swepub:gu)xklevs (författare)
Borén, Jan,1963Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,University of Gothenburg(Swepub:gu)xborej (författare)
Fletcher, John S.Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xfletj (författare)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

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Ingår i:International Journal of Mass Spectrometry: Elsevier BV437, s. 77-861387-3806

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Av författaren/redakt...: Sämfors, Sanna, ...; Ståhlman, Marcus ...; Klevstig, Martin ...; Borén, Jan, 1963; Fletcher, John S ...

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NATURVETENSKAP: NATURVETENSKAP; och Kemi; och Analytisk kemi

NATURVETENSKAP: NATURVETENSKAP; och Fysik

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Av lärosätet: Göteborgs universitet; Chalmers tekniska högskola

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