Artificial spider silk supports and guides neurite extension in vitro

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Artificial spider silk supports and guides neurite extension in vitro

Hansson, Magnus L. (author): Karolinska Inst, Dept Biosci & Nutr, S-14186 Huddinge, Sweden.;Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden.

Chatterjee, Urmimala (author): Karolinska Institutet

Francis, Juanita (author): Karolinska Inst, Dept Biosci & Nutr, S-14186 Huddinge, Sweden.

Arndt, Tina (author): Karolinska Institutet

Broman, Christian (author): Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden.

Johansson, Jan (author): Karolinska Institutet

Sköld, Mattias K. (author): Karolinska Institutet,Uppsala universitet,Enblad: Neurokirurgi,Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden.

Rising, Anna (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Karolinska Institutet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute

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Karolinska Institutet Karolinska Inst, Dept Biosci & Nutr, S-14186 Huddinge, Sweden;Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden. (creator_code:org_t)

John Wiley & Sons, 2021
2021
English.
In: The FASEB Journal. - : John Wiley & Sons. - 0892-6638 .- 1530-6860. ; 35:11

Related links:: https://doi.org/10.1...; show more...; https://uu.diva-port... (primary) (Raw object); https://pub.epsilon.... (primary) (Raw object) (free); https://urn.kb.se/re...; https://doi.org/10.1...; http://kipublication...; https://res.slu.se/i...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Surgical intervention with the use of autografts is considered the gold standard to treat peripheral nerve injuries. However, a biomaterial that supports and guides nerve growth would be an attractive alternative to overcome problems with limited availability, morbidity at the site of harvest, and nerve mismatches related to autografts. Native spider silk is a promising material for construction of nerve guidance conduit (NGC), as it enables regeneration of cm-long nerve injuries in sheep, but regulatory requirements for medical devices demand synthetic materials. Here, we use a recombinant spider silk protein (NT2RepCT) and a functionalized variant carrying a peptide derived from vitronectin (VN-NT2RepCT) as substrates for nerve growth support and neurite extension, using a dorsal root ganglion cell line, ND7/23. Two-dimensional coatings were benchmarked against poly-d-lysine and recombinant laminins. Both spider silk coatings performed as the control substrates with regards to proliferation, survival, and neurite growth. Furthermore, NT2RepCT and VN-NT2RepCT spun into continuous fibers in a biomimetic spinning set-up support cell survival, neurite growth, and guidance to an even larger extent than native spider silk. Thus, artificial spider silk is a promising biomaterial for development of NGCs.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)

Keyword

biomaterial
nerve guidance conduit
peripheral nerve
recombinant spider silk

Publication and Content Type

ref (subject category)
art (subject category)

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By the author/editor: Hansson, Magnus ...; Chatterjee, Urmi ...; Francis, Juanita; Arndt, Tina; Broman, Christia ...; Johansson, Jan; show more...; Sköld, Mattias K ...; Rising, Anna; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Medical Engineer ...; and Medical Material ...

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By the university: Uppsala University; Karolinska Institutet; Swedish University of Agricultural Sciences

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