Assessing the potential of 2D electrophoretic mobility spectroscopy (2D MOSY) for analytical applications

• Electrophoretic displacement of charged entity phase modulates the spectrum acquired in electrophoretic NMR experiments, and this modulation can be presented via 2D FT as 2D mobility spectroscopy (MOSY) spectra. We compare in various mixed solutions the chemical selectivity provided by 2D MOSY spectra with that provided by 2D diffusion-ordered spectroscopy (DOSY) spectra and demonstrate, under the conditions explored, a superior performance of the former method. 2D MOSY compares also favourably with closely related LC-NMR methods. The shape of 2D MOSY spectra in complex mixtures is strongly modulated by the pH of the sample, a feature that has potential for areas such as in drug discovery and metabolomics.

Subject headings

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

Keyword

NMR

H-1

electrophoretic NMR

2D DOSY NMR

electrophoretic mobility

self-diffusion

ion

acid

base

LC-NMRNMR

H-1

electrophoretic NMR

2D DOSY NMR

electrophoretic mobility

self-diffusion

ion

acid

base

LC-NMR

Kemi

Chemistry

Publication and Content Type

ref (subject category)

art (subject category)