Uptake, delivery, and anticancer activity of thymoquinone nanoparticles in breast cancer cells

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Fältnamn	Indikatorer	Metadata
000		03865naa a2200409 4500
001		oai:DiVA.org:uu-303298
003		SwePub
008		160915s2016 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3032982 URI
024	7	a https://doi.org/10.1007/s11051-016-3517-82 DOI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Fakhoury, Isabelleu Amer Univ Beirut, Dept Biol, Beirut, Lebanon.4 aut
245	1 0	a Uptake, delivery, and anticancer activity of thymoquinone nanoparticles in breast cancer cells
264		c 2016-07-25
264	1	b Springer Science and Business Media LLC,c 2016
338		a print2 rdacarrier
520		a Thymoquinone (TQ) is a promising anticancer molecule but its development is hindered by its limited bioavailability. Drug encapsulation is commonly used to overcome low drug solubility, limited bioavailability, and nonspecific targeting. In this project, TQ nanoparticles (TQ-NP) were synthesized and characterized. The cytotoxicity of the NP was investigated in nontumorigenic MCF-10-A breast cells, while the uptake, distribution, as well as the anticancer potential were investigated in MCF-7 and MDA-MB-231 breast cancer cells. Flash Nanoprecipitation and dynamic light scattering coupled with scanning electron microscopy were used to prepare and characterize TQ-NP prior to measuring their anticancer potential by MTT assay. The uptake and subcellular intake of TQ-NP were evaluated by fluorometry and confocal microscopy. TQ-NP were stable with a hydrodynamic average diameter size around 100 nm. Entrapment efficiency and loading content of TQ-NP were high (around 80 and 50 %, respectively). In vitro, TQ-NP had equal or enhanced anticancer activity effects compared to TQ in MCF-7 and aggressive MDA-MB-231 breast cancer cells, respectively, with no significant cytotoxicity of the blank NP. In addition, TQ and TQ-NP were relatively nontoxic to MCF-10-A normal breast cells. TQ-NP uptake mechanism was both time and concentration dependent. Treatment with inhibitors of endocytosis suggested the involvement of caveolin in TQ-NP uptake. This was further confirmed by subcellular localization findings showing the colocalization of TQ-NP with caveolin and transferrin as well as with the early and late markers of endocytosis. Altogether, the results describe an approach for the enhancement of TQ anticancer activity and uncover the mechanisms behind cell-TQ-NP interaction.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
653		a Thymoquinone
653		a Breast cancer
653		a Nanoparticles
653		a Uptake
653		a Endocytosis
700	1	a Saad, Walidu Amer Univ Beirut, Dept Chem & Petr Engn, Beirut, Lebanon.4 aut
700	1	a Bouhadir, Kamalu Amer Univ Beirut, Dept Chem, Beirut, Lebanon.4 aut
700	1	a Nygren, Peteru Uppsala universitet,Experimentell och klinisk onkologi4 aut0 (Swepub:uu)peterng
700	1	a Schneider-Stock, Regineu Univ Erlangen Nurnberg, Inst Pathol, Expt Tumor Pathol, Erlangen, Germany.4 aut
700	1	a Gali-Muhtasib, Halau Amer Univ Beirut, Dept Biol, Beirut, Lebanon.;Amer Univ Beirut, Dept Anat, Cell Biol, Physiol,Fac Med, Beirut, Lebanon.4 aut
710	2	a Amer Univ Beirut, Dept Biol, Beirut, Lebanon.b Amer Univ Beirut, Dept Chem & Petr Engn, Beirut, Lebanon.4 org
773	0	t Journal of nanoparticle researchd : Springer Science and Business Media LLCg 18:7q 18:7x 1388-0764x 1572-896X
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-303298
856	4 8	u https://doi.org/10.1007/s11051-016-3517-8

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Av författaren/redakt...: Fakhoury, Isabel ...; Saad, Walid; Bouhadir, Kamal; Nygren, Peter; Schneider-Stock, ...; Gali-Muhtasib, H ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Cancer och onkol ...

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