Sökning: WFRF:(Aguirre Javier) > tRNA tracking for d...
Fältnamn | Indikatorer | Metadata |
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000 | 03318naa a2200397 4500 | |
001 | oai:DiVA.org:uu-359663 | |
003 | SwePub | |
008 | 180905s2018 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3596632 URI |
024 | 7 | a https://doi.org/10.1038/s41589-018-0063-y2 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Volkov, Ivanu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)ivavo256 |
245 | 1 0 | a tRNA tracking for direct measurements of protein synthesis kinetics in live cells |
264 | c 2018-05-16 | |
264 | 1 | b NATURE PUBLISHING GROUP,c 2018 |
338 | a print2 rdacarrier | |
520 | a Our ability to directly relate results from test-tube biochemical experiments to the kinetics in living cells is very limited. Here we present experimental and analytical tools to directly study the kinetics of fast biochemical reactions in live cells. Dye-labeled molecules are electroporated into bacterial cells and tracked using super-resolved single-molecule microscopy.Trajectories are analyzed by machine-learning algorithms to directly monitor transitions between bound and free states. In particular, we measure the dwell time of tRNAs on ribosomes, and hence achieve direct measurements of translation rates inside living cells at codon resolution. We find elongation rates with tRNA(Phe) that are in perfect agreement with previous indirect estimates, and once fMet-tRNA(fMet) has bound to the 30S ribosomal subunit, initiation of translation is surprisingly fast and does not limit the overall rate of protein synthesis. The experimental and analytical tools for direct kinetics measurements in live cells have applications far beyond bacterial protein synthesis. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
700 | 1 | a Lindén, Martin,d 1976-u Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)marli467 |
700 | 1 | a Aguirre, Javieru Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)javag704 |
700 | 1 | a Ieong, Ka-Weng,d 1985-u Uppsala universitet,Molekylärbiologi4 aut0 (Swepub:uu)kawie779 |
700 | 1 | a Metelev, Mikhailu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)mikme204 |
700 | 1 | a Elf, Johanu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)johaande |
700 | 1 | a Johansson, Magnusu Uppsala universitet,Molekylär systembiologi4 aut0 (Swepub:uu)majoh087 |
710 | 2 | a Uppsala universitetb Molekylär systembiologi4 org |
773 | 0 | t Nature Chemical Biologyd : NATURE PUBLISHING GROUPg 14:6, s. 618-626q 14:6<618-626x 1552-4450x 1552-4469 |
856 | 4 | u https://europepmc.org/articles/pmc6124642?pdf=render |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-359663 |
856 | 4 8 | u https://doi.org/10.1038/s41589-018-0063-y |
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