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Potentiating Vγ9Vδ2...
Potentiating Vγ9Vδ2 T cell proliferation and assessing their cytotoxicity towards adherent cancer cells at the single cell level
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- Liu, Chenxiao (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Southern Univ Sci & Technol, Dept Infect Dis, Shenzhen 518020, Guangdong, Peoples R China.
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- Skorupinska-Tudek, K. (författare)
- Polish Acad Sci, Inst Biochem & Biophys, Lab Lipid Biochem, PL-02106 Warsaw, Poland.
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- Eriksson, Sven-Göran (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Univ Gothenburg, Dept Biomed, Sahlgrenska Acad, S-40530 Gothenburg, Sweden.
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- Parmryd, Ingela (författare)
- Uppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab,Univ Gothenburg, Dept Biomed, Sahlgrenska Acad, S-40530 Gothenburg, Sweden.
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(creator_code:org_t)
- 2022-02-07
- 2022
- Engelska.
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Ingår i: Biology Open. - : The Company of Biologists. - 2046-6390. ; 11:1
- Relaterad länk:
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Vγ9Vδ2 T cells is the dominant γδ T cell subset in human blood. They are cytotoxic and activated by phosphoantigens whose concentrations are increased in cancer cells, making the cancer cells targets for Vγ9Vδ2 T cell immunotherapy. For successful immunotherapy, it is important both to characterise Vγ9Vδ2 T cell proliferation and optimise the assessment of their cytotoxic potential, which is the aim of this study. We found that supplementation with freshly thawed human serum potentiated Vγ9Vδ2 T cell proliferation from peripheral mononuclear cells (PBMCs) stimulated with (E)-4-Hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) and consistently enabled Vγ9Vδ2 T cell proliferation from cryopreserved PBMCs. In cryopreserved PBMCs the proliferation was higher than in freshly prepared PBMCs. In a panel of short-chain prenyl alcohols, monophosphates and diphosphates, most diphosphates and also dimethylallylmonophosphate stimulated Vγ9Vδ2 T cell proliferation.We developed a method where the cytotoxicity of Vγ9Vδ2 T cells towards adherent cells is assessed at the single cell level using flow cytometry, which gives more clear-cut results than the traditional bulk release assays. Moreover, we found that HMBPP enhances the Vγ9Vδ2 T cell cytotoxicity towards colon cancer cells. In summary, we have developed an easily interpretable method to assess the cytotoxicity of Vγ9Vδ2 T cells towards adherent cells, found that Vγ9Vδ2 T cell proliferation can be potentiated by media-supplementation and how misclassification of non-responders may be avoided. Our findings will be useful in the further development of Vγ9Vδ2 T cell immunotherapy. © 2022. Published by The Company of Biologists Ltd.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- Colon cancer
- Cytotoxicity assay
- Phosphoantigens
- Proliferation
- Vγ9Vδ2 T cells
- Colon cancer
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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