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Sökning: L773:0197 3851 OR L773:1097 0223 > Workshop report on ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004049naa a2200541 4500
001oai:lup.lub.lu.se:98cef689-9203-411a-a7d0-aedb042b16da
003SwePub
008160401s2007 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/6558802 URI
0247 a https://doi.org/10.1002/pd.17832 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Legler, Tobias J.4 aut
2451 0a Workshop report on the extraction of foetal DNA from maternal plasma
264 c 2007
264 1b Wiley,c 2007
520 a Objective Cell free foetal DNA (cff DNA) extracted from maternal plasma is now recognized as a potential source for prenatal diagnosis but the methodology is currently not well standardized. To evaluate different manual and automated DNA extraction methods with a view to developing standards, an International Workshop was performed. Methods Three plasma pools from RhD-negative pregnant women, a DNA standard, real-time-PCR protocol, primers and probes for RHD were sent to 12 laboratories and also to one company (Qiagen, Hilden, Germany). In pre-tests, pool 3 showed a low cff DNA concentration, pool I showed a higher concentration and pool 2 an intermediate concentration. Results The QIAamp DSP Virus Kit, the High Pure PCR Template Preparation Kit, an in-house protocol using the QIAamp DNA Blood Mini Kit, the CST genomic DNA purification kit, the Magna Pure LC, the MDx, the M48, the EZl and an in-house protocol using magnetic beads for manual and automated extraction were the methods that were able to reliably detect foetal RHD. The best results were obtained with the QIAamp DSP Virus Kit. The QIAamp DNA Blood Mini Kit showed very comparable results in laboratories that followed the manufacturer's protocol and started with >= 500 mu L plasma. One participant using the QIAamp DNA Blood Midi Kit failed to detect reliably RHD in pool 3. Conclusions This workshop initiated a standardization process for extraction of cff DNA in maternal plasma. The highest yield was obtained by the QIAamp DSP Virus Kit, a result that will be evaluated in more detail in future studies. Copyright (c) 2007 John Wiley & Sons, Ltd.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reproduktionsmedicin och gynekologi0 (SwePub)302202 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Obstetrics, Gynaecology and Reproductive Medicine0 (SwePub)302202 hsv//eng
653 a cell-free foetal DNA
653 a non-invasive prenatal diagnosis
653 a maternal plasma
653 a genotyping
653 a RhD
653 a sensitivity
7001 a Liu, Zhong4 aut
7001 a Mavrou, Ariadni4 aut
7001 a Finning, Kirstin4 aut
7001 a Hromadnikova, Ilona4 aut
7001 a Galbiati, Silvia4 aut
7001 a Meaney, Cathy4 aut
7001 a Hulten, Maj A.4 aut
7001 a Crea, Francesco4 aut
7001 a Olsson, Martin Lu Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Transfusionsmedicin,Forskargrupper vid Lunds universitet,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine,Transfusion Medicine,Lund University Research Groups4 aut0 (Swepub:lu)labm-mol
7001 a Maddocks, Deborah G.4 aut
7001 a Huang, Dorothy4 aut
7001 a Fisher, Sylvia Armstrong4 aut
7001 a Sprenger-Haussels, Markus4 aut
7001 a Soussan, Aicha Ait4 aut
7001 a van der Schoot, C. Ellen4 aut
7102 a Avdelningen för hematologi och transfusionsmedicinb Institutionen för laboratoriemedicin4 org
7730 t Prenatal Diagnosisd : Wileyg 27:9, s. 824-829q 27:9<824-829x 1097-0223x 0197-3851
8564 u http://dx.doi.org/10.1002/pd.1783y FULLTEXT
8564 8u https://lup.lub.lu.se/record/655880
8564 8u https://doi.org/10.1002/pd.1783

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