Inner filter correction of dissolved organic matter fluorescence

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Fältnamn	Indikatorer	Metadata
000		03444naa a2200385 4500
001		oai:research.chalmers.se:16035264-957d-4215-a1ee-cb8aab5264ca
003		SwePub
008		171010s2013 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:uu-214176
024	7	a https://doi.org/10.4319/lom.2013.11.6162 DOI
024	7	a https://research.chalmers.se/publication/2116492 URI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2141762 URI
040		a (SwePub)cthd (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Kothawala, Dollyu Uppsala universitet,Limnologi4 aut0 (Swepub:uu)dolko444
245	1 0	a Inner filter correction of dissolved organic matter fluorescence
264		c 2013-12-03
264	1	b Wiley,c 2013
520		a The fluorescence of dissolved organic matter (DOM) is suppressed by a phenomenon of self-quenching known as the inner filter effect (IFE). Despite widespread use of fluorescence to characterize DOM in surface waters, the advantages and constraints of IFE correction are poorly defined. We assessed the effectiveness of a commonly used absorbance-based approach (ABA), and a recently proposed controlled dilution approach (CDA) to correct for IFE. Linearity between corrected fluorescence and total absorbance (ATotal; the sum of absorbance at excitation and emission wavelengths) across the full excitation-emission matrix (EEM) in dilution series of four samples indicated both ABA and CDA were effective to an absorbance of at least 1.5 in a 1 cm cell, regardless of wavelength positioning. In regions of the EEMs where signal to background noise (S/N) was low, CDA correction resulted in more variability than ABA correction. From the ABA algorithm, the onset of significant IFE (>5%) occurs when absorbance exceeds 0.042. In these cases, IFE correction is required, which was the case for the vast majority (97%) of lakes in a nationwide survey (n= 554). For highly absorbing samples, undesirably large dilution factors would be necessary to reduce absorbance below 0.042. For rare EEMs with ATotal > 1.5 (3.0% of the lakes in the Swedish survey), a 2-fold dilution is recommended followed by ABA or CDA correction. This study shows that for the vast majority of natural DOM samples the most commonly applied ABA algorithm provides adequate correction without prior dilution. © 2013, by the American Society of Limnology and Oceanography, Inc.
650	7	a NATURVETENSKAPx Geovetenskap och miljövetenskap0 (SwePub)1052 hsv//swe
650	7	a NATURAL SCIENCESx Earth and Related Environmental Sciences0 (SwePub)1052 hsv//eng
700	1	a Murphy, Kathleen,d 19724 aut
700	1	a Stedmon, C.A.4 aut
700	1	a Weyhenmeyer, Gesa A.u Uppsala universitet,Limnologi4 aut0 (Swepub:uu)gesaweyh
700	1	a Tranvik, Lars J.u Uppsala universitet,Limnologi4 aut0 (Swepub:uu)larstran
710	2	a Uppsala universitetb Limnologi4 org
773	0	t Limnology and Oceanography: Methodsd : Wileyg 11:DEC, s. 616-630q 11:DEC<616-630x 1541-5856
856	4	u http://dx.doi.org/10.4319/lom.2013.11.616y FULLTEXT
856	4	u https://aslopubs.onlinelibrary.wiley.com/doi/pdfdirect/10.4319/lom.2013.11.616
856	4 8	u https://doi.org/10.4319/lom.2013.11.616
856	4 8	u https://research.chalmers.se/publication/211649
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-214176

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Av författaren/redakt...: Kothawala, Dolly; Murphy, Kathleen ...; Stedmon, C.A.; Weyhenmeyer, Ges ...; Tranvik, Lars J.

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Av lärosätet: Chalmers tekniska högskola; Uppsala universitet

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