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WFRF:(Wang Ming Wei)
 

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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003359naa a2200541 4500
001oai:DiVA.org:umu-117443
003SwePub
008160229s2016 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1174432 URI
0247 a https://doi.org/10.1016/j.jhazmat.2015.12.0332 DOI
040 a (SwePub)umu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Qian, Yan4 aut
2451 0a Quantification for total demethylation potential of environmental samples utilizing the EGFP reporter gene
264 1b Elsevier,c 2016
338 a print2 rdacarrier
520 a Abstract The demethylation potential of pollutants is arguably an innate component of their toxicity in environmental samples. A method was developed for determining the total demethylation potential of food samples (TDQ). The demethylation epigenetic toxicity was determined using the Hep G2 cell line transfected with pEGFP-C3 plasmids containing a methylated promoter of the EGFP reporter gene. The total demethylation potential of the sample extracts (the 5-AZA-CdR demethylation toxic equivalency) can be quantified within one week by using a standard curve of the 5-AZA-CdR demethylation agent. To explore the applicability of TDQ for environmental samples, 17 groundwater samples were collected from heavy polluted Kuihe river and the total demethylation potentials of the sample extracts were measured successfully. Meaningful demethylation toxic equivalencies ranging from 0.00050 to 0.01747 μM were found in all groundwater sample extracts. Among 19 kinds of inorganic substance, As and Cd played important roles for individual contribution to the total demethylation epigenetic toxicity. The TDQ assay is reliable and fast for quantifying the DNA demethylation potential of environmental sample extracts, which may improve epigenetic toxicity evaluations for human risk assessment, and the consistent consuming of groundwater alongside the Kuihe river pose unexpected epigenetic health risk to the local residents.
650 7a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng
653 a Demethylation potential
653 a Environmental sample
653 a Kuihe river
653 a Epigenetic toxicity
653 a EGFP
7001 a Wang, Xiao-li4 aut
7001 a Lv, Zhan-lu4 aut
7001 a Tysklind, Matsu Umeå universitet,Kemiska institutionen4 aut0 (Swepub:umu)maty0001
7001 a Guo, Chen4 aut
7001 a Liang, Bao4 aut
7001 a Wu, Jia-bing4 aut
7001 a Yang, Yong-jian4 aut
7001 a Yang, Yi-shu4 aut
7001 a Wang, Fei-fei4 aut
7001 a Duan, Xiao-li4 aut
7001 a Ma, Jin4 aut
7001 a Wei, Yong-jie4 aut
7001 a Wang, Chun-hui4 aut
7001 a Yang, Li-xin4 aut
7001 a Zhang, Jin-liang4 aut
7001 a Shi, Xiao-ming4 aut
7001 a Wang, Xian-liang4 aut
7102 a Umeå universitetb Kemiska institutionen4 org
7730 t Journal of Hazardous Materialsd : Elsevierg 306, s. 278-285q 306<278-285x 0304-3894x 1873-3336
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-117443
8564 8u https://doi.org/10.1016/j.jhazmat.2015.12.033

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