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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003579naa a2200361 4500
001oai:prod.swepub.kib.ki.se:145434688
003SwePub
008240913s2020 | |||||||||||000 ||eng|
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1454346882 URI
0247 a https://doi.org/10.3389/fimmu.2020.5942432 DOI
040 a (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Yohannes, DA4 aut
2451 0a Effects of In Vivo Gluten Challenge on PBMC Gene Expression Profiles in Diet Treated Celiac Disease
264 c 2020-12-11
264 1b Frontiers Media SA,c 2020
520 a The pathological mechanisms that lead to the onset and reactivation of celiac disease (CD) remain largely unknown. While gluten free diet (GFD) improves the intestinal damage and associated clinical symptoms in majority of cases, it falls short of providing full recovery. Additionally, late or misdiagnosis is also common as CD presents with a wide range of symptoms. Clear understanding of CD pathogenesis is thus critical to address both diagnostic and treatment concerns. We aimed to study the molecular impact of short gluten exposure in GFD treated CD patients, as well as identify biological pathways that remain altered constitutively in CD regardless of treatment. Using RNAseq profiling of PBMC samples collected from treated CD patients and gluten challenged patient and healthy controls, we explored the peripheral transcriptome in CD patients following a short gluten exposure. Short gluten exposure of just three days was enough to alter the genome-wide PBMC transcriptome of patients. Pathway analysis revealed gluten-induced upregulation of mainly immune response related pathways, both innate and adaptive, in CD patients. We evaluated the perturbation of biological pathways in sample-specific manner. Compared to gluten exposed healthy controls, pathways related to tight junction, olfactory transduction, metabolism of unsaturated fatty acids (such as arachidonic acid), metabolism of amino acids (such as cysteine and glutamate), and microbial infection were constitutively altered in CD patients regardless of treatment, while GFD treatment appears to mostly normalize immune response pathways to “healthy” state. Upstream regulator prediction analysis using differentially expressed genes identified constitutively activated regulators relatively proximal to previously reported CD associated loci, particularly SMARCA4 on 19p13.2 and CSF2 on 5q31. We also found constitutively upregulated genes in CD that are in CD associated genetic loci such as MEF2BNB-MEF2B (BORCS8-MEF2B) on 19p13.11 and CSTB on 21q22.3. RNAseq revealed strong effects of short oral gluten challenge on whole PBMC fraction and constitutively altered pathways in CD PBMC suggesting important factors other than gluten in CD pathogenesis.
7001 a de Kauwe, A4 aut
7001 a Kaukinen, K4 aut
7001 a Kurppa, K4 aut
7001 a Maki, M4 aut
7001 a Anderson, RP4 aut
7001 a Linnarsson, Su Karolinska Institutet4 aut
7001 a Greco, D4 aut
7001 a Saavalainen, P4 aut
7102 a Karolinska Institutet4 org
7730 t Frontiers in immunologyd : Frontiers Media SAg 11, s. 594243-q 11<594243-x 1664-3224
8564 u https://www.frontiersin.org/articles/10.3389/fimmu.2020.594243/pdf
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:145434688
8564 8u https://doi.org/10.3389/fimmu.2020.594243

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