A complete workflow for the differentiation and the dissociation of hiPSC-derived cardiospheres

QC 20220509
Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) are an invaluable tool for both basic and translational cardiovascular research. The potential that these cells hold for therapy, disease modeling and drug discovery is hampered by several bottlenecks that currently limit both the yield and the efficiency of cardiac induction. Here, we present a complete workflow for the production of ready-to-use hiPSC-CMs in a dynamic suspension bioreactor. This includes the efficient and highly reproducible differentiation of hiPSCs into cardiospheres, which display enhanced physiological maturation compared to static 3D induction in hanging drops, and a novel papain-based dissociation method that offers higher yield and viability than the broadly used dissociation reagents TrypLE and Accutase. Molecular and functional analyses of the cardiomyocytes reseeded after dissociation confirmed both the identity and the functionality of the cells, which can be used in down-stream applications, either as monolayers or spheroids.

Meier, AnnaFraunhofer Inst Biomed Engn, Germany;Tech Univ Munich, Germany (författare)
Dehne, AnnikaFraunhofer Inst Biomed Engn, Germany (författare)
Salhotra, AseemLinnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Fraunhofer Inst Biomed Engn, Germany(Swepub:kth)u1bx7doc (författare)
Tran, Thao AnhFraunhofer Inst Biomed Engn, Germany (författare)
Neumann, SaschaFraunhofer Inst Biomed Engn, Germany;Univ Clin Essen, Germany (författare)
Schmidt, KatharinaFraunhofer Inst Biomed Engn, Germany (författare)
Meiser, InaFraunhofer Inst Biomed Engn, Germany (författare)
Neubauer, Julia C.Fraunhofer Inst Biomed Engn, Germany;Fraunhofer Project Ctr Stem Cell Proc Engn, Germany (författare)
Zimmermann, HeikoFraunhofer Inst Biomed Engn, Germany;Fraunhofer Project Ctr Stem Cell Proc Engn, Germany;Saarland Univ, Germany;Univ Catolica Norte, Chile (författare)
Gentile, LucaFraunhofer Inst Biomed Engn, Germany;Univ Appl Sci Kaiserslautern, Germany;Hasselt Univ, Belgium (författare)
Fraunhofer Inst Biomed Engn, GermanyFraunhofer Inst Biomed Engn, Germany;Tech Univ Munich, Germany (creator_code:org_t)

Av författaren/redakt...: Fischer, Benjami ...; Meier, Anna; Dehne, Annika; Salhotra, Aseem; Tran, Thao Anh; Neumann, Sascha; visa fler...; Schmidt, Kathari ...; Meiser, Ina; Neubauer, Julia ...; Zimmermann, Heik ...; Gentile, Luca; visa färre...

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