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Autoantibodies agai...
Autoantibodies against 21-hydroxylase and side-chain cleavage enzyme in autoimmune Addison's disease are mainly immunoglobulin G1
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- Boe, Anette S. (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)
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Bredholt, Geir (author)
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Knappskog, Per M. (author)
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Hjelmervik, Trond Ove (author)
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Mellgren, Gunnar (author)
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- Winqvist, Ola (author)
- Karolinska Institutet,Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)
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- Kämpe, Olle (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)
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Husebye, Eystein S. (author)
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(creator_code:org_t)
- Oxford University Press (OUP), 2004
- 2004
- English.
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In: European Journal of Endocrinology. - : Oxford University Press (OUP). - 0804-4643 .- 1479-683X. ; 150:1, s. 49-56
- Related links:
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http://www.ncbi.nlm....
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https://eje.bioscien...
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https://doi.org/10.1...
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Abstract
Subject headings
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- OBJECTIVE: Immunoglobulin G (IgG) antibodies to the steroidogenic enzymes 21-hydroxylase (21OH) and side-chain cleavage enzyme (SCC) are important diagnostic markers for autoimmune Addison's disease and autoimmune polyendocrine syndromes (APS) types I and II. The characterization of autoantibody (IgG) subclasses may reveal information on how tIssue destruction takes place; therefore, IgG subtypes of anti-21OH and anti-SCC antibodies from sera of patients with Addison's disease, APS I and APS II were determined using recombinant 21OH and SCC. METHODS: SCC(51-521) and his-SCC(51-521) were expressed by pET-scc in the Escherichia coli strain BL21 Star (DE3) and inclusion bodies were purified. Full-length, human 21OH fused to an N-terminal 6x histidine affinity tag was expressed in insect cells by using the baculovirus expression system bac-to-bac. Western blots were used to investigate the IgG subtype(s) of the autoantibodies against 21OH and SCC in patients and healthy blood donors. RESULTS: All anti-SCC positive sera (n=10) contained autoantibodies of the IgG1 subclass, while four out of ten also contained IgG3. All anti-21OH positive sera (n=16) had autoantibodies exclusively against IgG1. Sera from 20 healthy subjects did not show any reactivity against 21OH or SCC. CONCLUSIONS: The finding of a predominating IgG1 response against 21OH and SCC may suggest that T helper (Th) cells of the Th1 subclass are involved in destruction of the adrenal cortex in patients with autoimmune Addison's disease.
Keyword
- Addison Disease/*immunology
- Antibody Specificity
- Autoantibodies/blood/*immunology
- Biological Markers
- Cholesterol Side-Chain Cleavage Enzyme/genetics/*immunology
- Gene Expression Regulation; Enzymologic
- Humans
- Immunoglobulin G/blood/immunology
- Research Support; Non-U.S. Gov't
- Steroid 21-Hydroxylase/genetics/*immunology
- T-Lymphocytes/immunology
- MEDICINE
- MEDICIN
Publication and Content Type
- vet (subject category)
- art (subject category)
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