Search: WFRF:(Erlanson M.) >
mRNA for pancreatic...
mRNA for pancreatic uncoupling protein 2 increases in two models of acute experimental pancreatitis in rats and mice
-
- Segersvard, R (author)
- Karolinska Institutet
-
- Rippe, Catarina (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
-
DuPlantier, M (author)
-
show more...
-
Herrington, MK (author)
-
- Isaksson, B (author)
- Karolinska Institutet
-
Adrian, TE (author)
-
- Erlanson-Albertsson, Charlotte (author)
- Lund University,Lunds universitet,Aptitkontroll,Forskargrupper vid Lunds universitet,Appetite Regulation,Lund University Research Groups
-
- Permert, J (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2005-03-22
- 2005
- English.
-
In: Cell and Tissue Research. - : Springer Science and Business Media LLC. - 1432-0878 .- 0302-766X. ; 320:2, s. 251-258
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Uncoupling-protein 2 (UCP2) is a mitochondrial protein that appears to be involved in cellular oxidant defense and in the regulation of oncotic cell death, both of which are important features of acute pancreatitis. However, UCP2 expression in acute pancreatitis has not been previously reported. In the current experiments, pancreatic gene expression was studied by real-time reverse-transcription/polymerase chain reaction and Northern blots. Two models of acute experimental pancreatitis were investigated: cerulein-induced pancreatitis in mice at two different time points and taurocholate-induced pancreatitis in rats at two degrees of severity. After cerulein administration, acinar injury and leukocyte infiltration was significantly higher at 24 h compared with 12 h after the first injection of cerulein (P < 0.05, P < 0.005, respectively). UCP2 mRNA was unchanged at 12 h but was nearly 12-fold greater than control levels after 24 h (P < 0.001). UCP2 gene expression correlated with acinar injury (r=0.69; P < 0.001). By 72 h after taurocholate administration, the severe group had more necrosis than the mild group (P < 0.005). Pancreatic UCP2 mRNA was increased fourfold in the severe group compared with controls (P < 0.01). UCP2 expression correlated with parenchymal necrosis (r=0.61; P < 0.01). Thus, pancreatic UCP2 mRNA increased in two models of acute pancreatitis. The increase in UCP2 gene expression was correlated with the severity of the disease. Up-regulation of UCP2 in the pancreas may be a protective response to oxidative stress, but this increase may also have a negative influence on cellular energy metabolism. Therefore, acinar UCP2 may be an important modifier of the severity of acute pancreatitis.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- uncoupling proteins
- acute pancreatitis
- oxidative stress
- rat (male Zucker)
- mouse
- UCP2
- (female C57/B16)
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database