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Three-dimensional b...
Three-dimensional bioprinting using a coaxial needle with viscous inks in bone tissue engineering - An in vitro study
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- Walladbegi, Java (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology,Department of Oral and Maxillofacial Surgery, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Schaefer, Christian (författare)
- Göteborgs universitet,University of Gothenburg,Department of Oral and Maxillofacial Surgery, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Pernevik, Elin, 1989 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Wallenberg Wood Science Center, Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden
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- Sämfors, Sanna, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Wallenberg Wood Science Center, Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden
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- Kjeller, Göran (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology,Department of Oral and Maxillofacial Surgery, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Gatenholm, Paul, 1956 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Wallenberg Wood Science Center, Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden
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- Sándor, George K. (författare)
- Oulun Yliopisto,University of Oulu,Department of Oral and Maxillofacial Surgery, Medical Research Center, University of Oulu, Oulu University Hospital, Oulu, Finland
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- Rasmusson, Lars, 1962 (författare)
- Linköpings universitet,Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology,Avdelningen för sinnesorgan och kommunikation,Medicinska fakulteten,Region Östergötland, Käkkliniken US,University of Gothenburg, Sweden
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(creator_code:org_t)
- Medknow, 2020
- 2020
- Engelska.
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Ingår i: Annals of Maxillofacial Surgery. - : Medknow. - 2249-3816 .- 2231-0746. ; 10:2, s. 370-376
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Abstract
Ämnesord
Stäng
- Introduction: Vascularized autologous tissue grafts are considered 'gold standard' for the management of larger bony defects in the craniomaxillofacial area. This modality does however carry limitations, such as the absolute requirement for healthy donor tissues and recipient vessels. In addition, the significant morbidity of large bone graft is deterrent to fibula bone flap use. Therefore, less morbid strategies would be beneficial. The purpose of this study was to develop a printing method to manufacture scaffold structure with viable stem cells. Materials and Methods: In total, three different combinations of ground beta tri-calcium phosphate and CELLINK (bioinks) were printed with a nozzle to identify a suitable bioink for three-dimensional printing. Subsequently, a coaxial needle, with three different nozzle gauge combinations, was evaluated for printing of the bioinks. Scaffold structures (grids) were then printed alone and with additional adipose stem cells before being transferred into an active medium and incubated overnight. Following incubation, grid stability was evaluated by assessing the degree of maintained grid outline, and cell viability was determined using the live/dead cell assay. Results: Among the three evaluated combinations of bioinks, two resulted in good printability for bioprinting. Adequate printing was obtained with two out of the three nozzle gauge combinations tested. However, due to the smaller total opening, one combination revealed a better stability. Intact grids with maintained stability were obtained using Ink B23 and Ink B42, and approximately 80% of the printed stem cells were viable following 24 hours. Discussion: Using a coaxial needle enables printing of a stable scaffold with viable stem cells. Furthermore, cell viability is maintained after the bioprinting process.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- viability
- in vitro
- CELLINK
- Adipose stem cells
- bioinks
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Walladbegi, Java
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Schaefer, Christ ...
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Pernevik, Elin, ...
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Sämfors, Sanna, ...
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Kjeller, Göran
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Gatenholm, Paul, ...
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Sándor, George K ...
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Rasmusson, Lars, ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Biomaterialveten ...
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TEKNIK OCH TEKNO ...
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och Medicinsk materi ...
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Annan industriel ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Kirurgi
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Annals of Maxill ...
- Av lärosätet
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Chalmers tekniska högskola
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Göteborgs universitet
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Linköpings universitet