The establishment of 20 different human embryonic stem cell lines and subclones; a report on derivation, culture, characterisation and banking.

• This report summarises our efforts in deriving, characterising and banking of 20 different human embryonic stem cell lines. We have derived a large number of human embryonic stem cell lines between 2001 and 2005. One of these cell lines was established under totally xeno-free culture conditions. In addition, several subclones have been established, including a karyoptypical normal clone from a trisomic mother line. A master cell banking system has been utilised in concert with an extensive characterisation programme, ensuring a supply of high quality pluripotent stem cells for further research and development. In this report we also present the first data on a proprietary novel antibody, hES-Cellect, that exhibits high specificity for undifferentiated hES cells. In addition to the traditional manual dissection approach of propagating hES cells, we here also report on the successful approaches of feeder-free cultures as well as single cell cultures based on enzymatic digestion. All culture systems used as reported here have maintained the hES cells in a karyotypical normal and pluripotent state. These systems also have the advantage of being the principal springboards for further scale up of cultures for industrial or clinical applications that would require vastly more cells that can be produced by mechanical means.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Keyword

Animals

Cell Culture Techniques

methods

Cell Differentiation

drug effects

Cell Line

Clone Cells

cytology

Collagen

pharmacology

Dissection

Drug Combinations

Embryonic Stem Cells

cytology

drug effects

metabolism

Humans

Immunohistochemistry

In Situ Hybridization

Fluorescence

Karyotyping

Laminin

pharmacology

Mice

Proteoglycans

pharmacology

Quality Control

Telomerase

metabolism

Tissue Banks

Cell banking

Characterisation

Pluripotency

Single cells

Feeder-free

Xeno-free

hES cells

Derivation

Publication and Content Type

ref (subject category)

art (subject category)