Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

Xu, Hui (author): State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China

Li, Zhen (author): Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium

Jiang, Peng-Fei (author): State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China

Zhao, Li (author): Umeå universitet,Institutionen för ekologi, miljö och geovetenskap,UMFpub

Qu, Chang (author): State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China

Van de Peer, Yves (author): Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium; Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa

Liu, Yan-Jing (author): State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China

Zeng, Qing-Yin (author): State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China

(creator_code:org_t)

2022-02-22
2022
English.
In: The Plant Journal. - : John Wiley & Sons. - 0960-7412 .- 1365-313X. ; 110:1, s. 129-146

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributions of active site motifs to the functional divergence of duplicate genes have not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in the Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.

By the author/editor: Xu, Hui; Li, Zhen; Jiang, Peng-Fei; Zhao, Li; Qu, Chang; Van de Peer, Yve ...; show more...; Liu, Yan-Jing; Zeng, Qing-Yin; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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