Sökning: L773:1545 9985 OR L773:1545 9993 >
Gating interaction ...
Gating interaction maps reveal a noncanonical electromechanical coupling mode in the Shaker K+ channel
-
- Fernandez-Marino, Ana I. (författare)
- Univ Wisconsin, SMPH, Dept Neurosci, Madison, WI 53706 USA.;NINDS, Mol Physiol & Biophys Sect, Porter Neurosci Res Ctr, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
-
- Harpole, Tyler J. (författare)
- KTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab
-
- Oelstrom, Kevin (författare)
- Univ Wisconsin, SMPH, Dept Neurosci, Madison, WI 53706 USA.;Cellular Dynam Int Inc, Madison, WI USA.
-
visa fler...
-
- Delemotte, Lucie (författare)
- KTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab
-
- Chanda, Baron (författare)
- Univ Wisconsin, SMPH, Dept Neurosci, Madison, WI 53706 USA.;Univ Wisconsin, SMPH, Dept Biomol Chem, Madison, WI 53706 USA.
-
visa färre...
-
Univ Wisconsin, SMPH, Dept Neurosci, Madison, WI 53706 USA;NINDS, Mol Physiol & Biophys Sect, Porter Neurosci Res Ctr, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. Tillämpad fysik (creator_code:org_t)
- 2018-03-26
- 2018
- Engelska.
-
Ingår i: Nature Structural & Molecular Biology. - : Nature Publishing Group. - 1545-9993 .- 1545-9985. ; 25:4, s. 320-326
- Relaterad länk:
-
https://europepmc.or...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Membrane potential regulates the activity of voltage-dependent ion channels via specialized voltage-sensing modules, but the mechanisms involved in coupling voltage-sensor movement to pore opening remain unclear owing to a lack of resting state structures and robust methods to identify allosteric pathways. Here, using a newly developed interaction-energy analysis, we probe the interfaces of the voltage-sensing and pore modules in the Drosophila Shaker K+ channel. Our measurements reveal unexpectedly strong equilibrium gating interactions between contacts at the S4 and S5 helices in addition to those between S6 and the S4-S5 linker. Network analysis of MD trajectories shows that the voltage-sensor and pore motions are linked by two distinct pathways: a canonical pathway through the S4-S5 linker and a hitherto unknown pathway akin to rack-and-pinion coupling involving the S4 and S5 helices. Our findings highlight the central role of the S5 helix in electromechanical transduction in the voltage-gated ion channel (VGIC) superfamily.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas