Sökning: WFRF:(Eliasson M) > (2020-2024) > The structure of in...
Fältnamn | Indikatorer | Metadata |
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000 | 06290nam a2200421 4500 | |
001 | oai:lup.lub.lu.se:d3f28b54-80d1-4465-a020-cc342db0ea41 | |
003 | SwePub | |
008 | 221007s2022 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/d3f28b54-80d1-4465-a020-cc342db0ea412 URI |
024 | 7 | a https://doi.org/10.1101/2022.02.22.4814552 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ovr2 swepub-publicationtype |
072 | 7 | a vet2 swepub-contenttype |
100 | 1 | a Barghouth, Mohammadu Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups4 aut0 (Swepub:lu)mo4503ba |
245 | 1 0 | a The structure of insulin granule core determines secretory capacity being reduced in type-2 diabetes |
264 | 1 | b Cold Spring Harbor Laboratory,c 2022 |
520 | a Exocytosis in excitable cells is essential for their physiological functions. Although the exocytotic machinery controlling cellular secretion has been well investigated, the function of the vesicular cargo, i.e. secretory granular content remains obscure. Here we combine dSTORM imaging and single-domain insulin antibody, to dissect the in situ structure of insulin granule cores (IGCs) at nano level. We demonstrate that the size and shape of the IGCs can be regulated by the juxta-granular molecules Nucleobindin-2 and Enolase-1, that further contribute to the stimulated insulin secretion. IGCs located at the plasma membrane are larger than those in the cytosol. The IGCs size is decreased by ∼20% after glucose stimulation due to the release of the peripheral part of IGCs through incomplete granule fusion. Importantly, the reduction of the IGCs size is also observed in non-stimulatory pancreatic β-cells from diabetic db/db mice, Akita (Ins2+/-) mice and human Type-2 diabetic donors, in accordance with impaired secretion. These findings overall highlight the structure of exocytotic insulin cores as a novel modality amenable to targeting in the stimulated exocytosis in β-cells with impaired insulin secretion.Competing Interest StatementThe authors have declared no competing interest. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng |
700 | 1 | a Jiang, Xiaopingu Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups,Southwest University4 aut0 (Swepub:lu)xi4613ji |
700 | 1 | a Nagao, Mototsuguu Lund University,Lunds universitet,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Diabetes - Islet Cell Exocytosis,Lund University Research Groups4 aut0 (Swepub:lu)med-mn9 |
700 | 1 | a Chen, Ningu Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups,Fudan University4 aut0 (Swepub:lu)ni0408ch |
700 | 1 | a Yang, Daoweiu Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,Other operations, LTH,Faculty of Engineering, LTH,Diabetes - Islet Patophysiology,Lund University Research Groups,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH4 aut0 (Swepub:lu)da7247ya |
700 | 1 | a Ye, Yingying4 aut |
700 | 1 | a Luan, Chengu Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups4 aut0 (Swepub:lu)med-cgl |
700 | 1 | a Gomez, Maria F.u Lund University,Lunds universitet,Diabetiska komplikationer,Forskargrupper vid Lunds universitet,Diabetic Complications,Lund University Research Groups4 aut0 (Swepub:lu)mphy-mgo |
700 | 1 | a Blom, Anna M.u Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Proteinkemi, Malmö,Forskargrupper vid Lunds universitet,Department of Translational Medicine,Faculty of Medicine,Protein Chemistry, Malmö,Lund University Research Groups4 aut0 (Swepub:lu)klke-abl |
700 | 1 | a Wollheim, Claes Bu Lund University,Lunds universitet,Diabetiska komplikationer,Forskargrupper vid Lunds universitet,Diabetic Complications,Lund University Research Groups,Geneva University Hospital4 aut0 (Swepub:lu)med-cwe |
700 | 1 | a Eliasson, Lenau Lund University,Lunds universitet,Medicinska fakulteten,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Faculty of Medicine,Diabetes - Islet Cell Exocytosis,Lund University Research Groups4 aut0 (Swepub:lu)mphy-lel |
700 | 1 | a Renström, Eriku Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,Lunds Tekniska Högskola,Diabetes - Islet Patophysiology,Lund University Research Groups,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH4 aut0 (Swepub:lu)mphy-ere |
700 | 1 | a Zhang, Enmingu Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,Other operations, LTH,Faculty of Engineering, LTH,Diabetes - Islet Patophysiology,Lund University Research Groups,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH4 aut0 (Swepub:lu)med-ezg |
710 | 2 | a Diabetes - öpatofysiologib Forskargrupper vid Lunds universitet4 org |
856 | 4 | u http://dx.doi.org/10.1101/2022.02.22.481455x freey FULLTEXT |
856 | 4 | u https://www.biorxiv.org/content/biorxiv/early/2022/02/23/2022.02.22.481455.full.pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/d3f28b54-80d1-4465-a020-cc342db0ea41 |
856 | 4 8 | u https://doi.org/10.1101/2022.02.22.481455 |
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