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Enhancing a de novo...
Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening
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- Risso, Valeria A. (author)
- Univ Granada, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Fac Ciencias, Dept Quim Fis, Granada 18071, Spain
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- Romero-Rivera, Adrian (author)
- Uppsala universitet,Biokemi,Science for Life Laboratory, SciLifeLab,Kamerlin
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- Gutierrez-Rus, Luis I. (author)
- Univ Granada, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Fac Ciencias, Dept Quim Fis, Granada 18071, Spain
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- Ortega-Muñoz, Mariano (author)
- Univ Granada, Dept Quim Organ, Fac Ciencias, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada 18071, Spain
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- Santoyo-Gonzalez, Francisco (author)
- Univ Granada, Dept Quim Organ, Fac Ciencias, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Granada 18071, Spain
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- Gavira, Jose A. (author)
- Univ Granada, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Inst Andaluz Ciencias Tierra, Lab Estudios Cristalog,CSIC, Ave Palmeras 4, Granada 18100, Spain
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- Sanchez-Ruiz, Jose M. (author)
- Univ Granada, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Fac Ciencias, Dept Quim Fis, Granada 18071, Spain
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- Kamerlin, Shina C. L., 1981- (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Biokemi,Kamerlin
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(creator_code:org_t)
- 2020
- 2020
- English.
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In: Chemical Science. - : Royal Society of Chemistry (RSC). - 2041-6520 .- 2041-6539. ; 11:24, s. 6134-6148
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Abstract
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- Directed evolution has revolutionized protein engineering. Still, enzyme optimization by random library screening remains sluggish, in large part due to futile probing of mutations that are catalytically neutral and/or impair stability and folding. FuncLib is a novel approach which uses phylogenetic analysis and Rosetta design to rank enzyme variants with multiple mutations, on the basis of predicted stability. Here, we use it to target the active site region of a minimalist-designed, de novo Kemp eliminase. The similarity between the Michaelis complex and transition state for the enzymatic reaction makes this system particularly challenging to optimize. Yet, experimental screening of a small number of active-site variants at the top of the predicted stability ranking leads to catalytic efficiencies and turnover numbers (∼2 × 104 M−1 s−1 and ∼102 s−1) for this anthropogenic reaction that compare favorably to those of modern natural enzymes. This result illustrates the promise of FuncLib as a powerful tool with which to speed up directed evolution, even on scaffolds that were not originally evolved for those functions, by guiding screening to regions of the sequence space that encode stable and catalytically diverse enzymes. Empirical valence bond calculations reproduce the experimental activation energies for the optimized eliminases to within ∼2 kcal mol−1 and indicate that the enhanced activity is linked to better geometric preorganization of the active site. This raises the possibility of further enhancing the stability-guidance of FuncLib by computational predictions of catalytic activity, as a generalized approach for computational enzyme design.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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