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  • Magnusson, Stefan (författare)

Release of pig leukocytes during pig kidney perfusion and characterization of pig lymphocyte carbohydrate xenoantigens.

  • Artikel/kapitelEngelska2003

Förlag, utgivningsår, omfång ...

  • 2003

Nummerbeteckningar

  • LIBRIS-ID:oai:gup.ub.gu.se/211614
  • https://gup.ub.gu.se/publication/211614URI

Kompletterande språkuppgifter

  • Språk:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The Galalpha1-3Gal (alphaGal) antigen is considered the main xenoantigen in the pig to human species combination but other porcine antigens have to be considered such as the swine lymphocyte antigen (SLA), the blood group A/O and the Hanganutziu-Deicher (H-D) antigens. The H-D antigens are N-glycolyl-neuraminic acid (NeuGc) terminated gangliosides that are widely distributed in mammalian species but absent in humans. Upon exposure to a vascularized pig organ, the human recipient can be immunized by direct interaction with the pig tissue or/and by transfer of tissue/cells from the organ into the recipient. In the present work, we describe the release of cells from porcine kidneys upon perfusion and the expression of glycolipid based alphaGal, blood group A/O and H-D antigens in pig lymphocytes. Pig kidneys were flushed with 20 ml of NaCl or Lidocain containing 5000 U heparin, and thereafter perfused with 3000-ml perfusion solution and the cells released were counted and examined microscopically. Neutral glycolipid and ganglioside fractions were extracted from purified pig lymphocytes. The extracted components were characterized by thin layer chromatography, degradation and mass spectrometry. The expression of alphaGal and H-D epitopes on cells released from pig kidneys and purified pig lymphocytes were studied by immune electron microscopy. A total amount of about 300 x 106 leukocytes, mainly lymphocytes were released in the perfusate from the kidneys, of which about 100 x 106 cells were eluated in the 600 to 2400 ml perfusate fraction. Immunelectron microscopical analysis with Griffonia simplicifolia isolectin B4 showed staining of pig leukocytes and other cells, morphologically similar to endothelial cells, released in the perfusate. The purified porcine lymphocytes contained 930 microg neutral glycolipid (4.2 microg/mg cell protein) of which 95% was glycolipids with one to four sugar residues. Immunostaining of the neutral glycolipid fractions revealed alphaGal terminated compounds migrating in the five and 10 to 12 sugar regions and blood group A compounds in the six and eight sugar regions. Two major gangliosides NeuGc-GM3 and NeuGc-GD3 were found in the pig lymphocytes. In a patient extracorporeally xenoperfused with a pig kidney, an increased staining of both alphaGal terminated structures as well as the H-D reactive gangliosides were found in the post-perfusion serum samples. In summary, leukocytes, mainly lymphocytes are released from pig kidneys during perfusion which may contribute to immunization of human xenograft recipients.

Ämnesord och genrebeteckningar

  • MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kirurgi hsv//swe
  • MEDICAL AND HEALTH SCIENCES Clinical Medicine Surgery hsv//eng
  • ABO Blood-Group System
  • immunology
  • Animals
  • Antigens
  • Heterophile
  • analysis
  • Carbohydrate Sequence
  • Cell Separation
  • methods
  • Chromatography
  • Thin Layer
  • Female
  • Gangliosides
  • chemistry
  • isolation & purification
  • Glycolipids
  • isolation & purification
  • Heparin
  • Humans
  • Kidney
  • blood supply
  • Leukocytes
  • physiology
  • Lidocaine
  • Lymphocytes
  • chemistry
  • cytology
  • immunology
  • Male
  • Mass Spectrometry
  • Microscopy
  • Immunoelectron
  • Molecular Sequence Data
  • Organ Preservation
  • methods
  • Perfusion
  • Swine

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Månsson, Jan-Eric (författare)
  • Strokan, Valeri (författare)
  • Jussila, Rainer (författare)
  • Kobayashi, Takaaki (författare)
  • Rydberg, Lennart,1944Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine(Swepub:gu)xrydle (författare)
  • Romano, Egidio (författare)
  • Breimer, Michael,1951Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery(Swepub:gu)xbremi (författare)
  • Göteborgs universitetInstitutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Xenotransplantation10:5, s. 432-450908-665X

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