Genome-scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: WFRF:(Kerkhoven Eduard 1985) > Genome-scale metabo...

LIBRIS Formathandbok (Information om MARC21)

Fältnamn	Indikatorer	Metadata
000		03223naa a2200397 4500
001		oai:research.chalmers.se:54d89f51-9ed2-4b10-827e-2f7095f6a376
003		SwePub
008		171007s2016 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://doi.org/10.1002/bit.258632 DOI
024	7	a https://research.chalmers.se/publication/2357442 URI
040		a (SwePub)cth
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Irani, Z. A.u Tarbiat Modares University4 aut
245	1 0	a Genome-scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins
264		c 2015-11-02
264	1	b Wiley,c 2016
338		a electronic2 rdacarrier
520		a Pichia pastoris is used for commercial production of human therapeutic proteins, and genome-scale models of P. pastoris metabolism have been generated in the past to study the metabolism and associated protein production by this yeast. A major challenge with clinical usage of recombinant proteins produced by P. pastoris is the difference in N-glycosylation of proteins produced by humans and this yeast. However, through metabolic engineering, a P. pastoris strain capable of producing humanized N-glycosylated proteins was constructed. The current genome-scale models of P. pastoris do not address native nor humanized N-glycosylation, and we therefore developed ihGlycopastoris, an extension to the iLC915 model with both native and humanized N-glycosylation for recombinant protein production, but also an estimation of N-glycosylation of P. pastoris native proteins. This new model gives a better prediction of protein yield, demonstrates the effect of the different types of N-glycosylation of protein yield, and can be used to predict potential targets for strain improvement. The model represents a step towards a more complete description of protein production in P. pastoris, which is required for using these models to understand and optimize protein production processes.
650	7	a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
653		a Pichia pastoris
653		a genome scale metabolic model
653		a humanized glycosylation
653		a recombinant protein production
700	1	a Kerkhoven, Eduard,d 1985u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)eduardk
700	1	a Shojaosadati, S. A.u Tarbiat Modares University4 aut
700	1	a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nielsenj
710	2	a Tarbiat Modares Universityb Chalmers tekniska högskola4 org
773	0	t Biotechnology and Bioengineeringd : Wileyg 113:5, s. 961-969q 113:5<961-969x 0006-3592x 1097-0290
856	4	u http://publications.lib.chalmers.se/records/fulltext/235744/local_235744.pdfx primaryx freey FULLTEXT
856	4	u http://publications.lib.chalmers.se/records/fulltext/235744/local_235744.pdf
856	4 8	u https://doi.org/10.1002/bit.25863
856	4 8	u https://research.chalmers.se/publication/235744

Hitta via bibliotek

Biotechnology and Bioengineering (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Irani, Z. A.; Kerkhoven, Eduar ...; Shojaosadati, S. ...; Nielsen, Jens B, ...

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Mikrobiologi

Artiklar i publikationen: Biotechnology an ...

Av lärosätet: Chalmers tekniska högskola

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se