Chemotactic motility is required for invasion of the host by the fish pathogen Vibrio anguillarum.

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O'Toole, R (författare)

Chemotactic motility is required for invasion of the host by the fish pathogen Vibrio anguillarum.

Artikel/kapitelEngelska1996

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1996
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LIBRIS-ID:oai:DiVA.org:umu-121654
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-121654URI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:art swepub-publicationtype

Anmärkningar

The role of the flagellum and motility in the virulence of the marine fish pathogen Vibrio anguillarum was examined. Non-motile mutants were generated by transposon mutagenesis. Infectivity studies revealed that disruption of the flagellum and subsequent loss of motility correlated with an approximate 500-fold decrease in virulence when fish were inoculated by immersion in bacteria-containing water. However, the flagellar filament and motility were not required for pathogenicity following intraperitoneal injection of fish. The transposon-insertion site for six mutants was determined by cloning and sequencing of the Vibrio DNA flanking the transposon. V. anguillarum genes whose products showed strong homology to proteins with an established role in flagellum biosynthesis were identified. One of the aflagellate mutants had a transposon insertion in the rpoN gene of V. anguillarum. This rpoN mutant failed to grow at low concentrations of available iron and was avirulent by both the immersion and intraperitoneal modes of inoculation. A chemotaxis gene, cheR, was located upstream of one transposon insertion and an in-frame deletion was constructed in the coding region of this gene. The resulting non-chemotactic mutant exhibited wild-type pathogenicity when injected intra-peritoneally into fish but showed a decrease in virulence similar to that seen for the non-motile aflagellate mutants following immersion infection. Hence, chemotactic motility is a required function of the flagellum for the virulence of V. anguillarum.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng

Biuppslag (personer, institutioner, konferenser, titlar ...)

Milton, Debra LUmeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet) (författare)
Wolf-Watz, H (författare)
Umeå universitetInstitutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet) (creator_code:org_t)

Sammanhörande titlar

Ingår i:Molecular Microbiology19:3, s. 625-6370950-382X1365-2958

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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-121654

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Molecular Microbiology (Sök värdpublikationen i LIBRIS)

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Av författaren/redakt...: O'Toole, R; Milton, Debra L; Wolf-Watz, H

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Mikrobiologi

Artiklar i publikationen: Molecular Microb ...

Av lärosätet: Umeå universitet

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