Search: WFRF:(Uhlen M) >
PDGFB, a new candid...
PDGFB, a new candidate plasma biomarker for venous thromboembolism : results from the VEREMA affinity proteomics study
-
- Bruzelius, Maria (author)
- Karolinska Institutet
-
- Iglesias, Maria Jesus (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Hong, Mun-Gwan (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
show more...
-
- Sanchez-Rivera, Laura (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
Gyorgy, Beata (author)
-
Carlos Souto, Juan (author)
-
- Frånberg, Mattias (author)
- Stockholms universitet,Numerisk analys och datalogi (NADA),Karolinska Institutet, Sweden
-
- Fredolini, Claudia (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Strawbridge, Rona J. (author)
- Karolinska Institutet
-
- Holmström, Margareta (author)
- Karolinska Institutet
-
- Hamsten, Anders (author)
- Karolinska Institutet
-
- Uhlén, Mathias (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Silveira, Angela (author)
- Karolinska Institutet
-
Manuel Soria, Jose (author)
-
Smadja, David M. (author)
-
- Butler, Lynn M. (author)
- Karolinska Institutet
-
- Schwenk, Jochen M. (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
Morange, Pierre-Emmanuel (author)
-
Tregouet, David-Alexandre (author)
-
- Odeberg, Jacob (author)
- Karolinska Institutet,KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Karolinska University Hospital, Sweden; Karolinska Institutet, Sweden
-
show less...
-
(creator_code:org_t)
- American Society of Hematology, 2016
- 2016
- English.
-
In: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 128:23, s. E59-E66
- Related links:
-
https://ashpublicati...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- There is a clear clinical need for high-specificity plasma biomarkers for predicting risk of venous thromboembolism (VTE), but thus far, such markers have remained elusive. Utilizing affinity reagents from the Human Protein Atlas project and multiplexed immuoassays, we extensively analyzed plasma samples from 2 individual studies to identify candidate protein markers associated with VTE risk. We screened plasma samples from 88 VTE cases and 85 matched controls, collected as part of the Swedish Venous Thromboembolism Biomarker Study, using suspension bead arrays composed of 755 antibodies targeting 408 candidate proteins. We identified significant associations between VTE occurrence and plasma levels of human immunodeficiency virus type I enhancer binding protein 1 (HIVEP1), von Willebrand factor (VWF), glutathione peroxidase 3 (GPX3), and platelet-derived growth factor beta (PDGFB). For replication, we profiled plasma samples of 580 cases and 589 controls from the French FARIVE study. These results confirmed the association of VWF and PDGFB with VTE after correction for multiple testing, whereas only weak trends were observed for HIVEP1 and GPX3. Although plasma levels of VWF and PDGFB correlated modestly (rho similar to 0.30) with each other, they were independently associated with VTE risk in a joint model in FARIVE (VWF P < .001; PDGFB P = .002). PDGF. was verified as the target of the capture antibody by immunocapture mass spectrometry and sandwich enzyme-linked immunosorbent assay. In conclusion, we demonstrate that high-throughput affinity plasma proteomic profiling is a valuable research strategy to identify potential candidate biomarkers for thrombosis-related disorders, and our study suggests a novel association of PDGFB plasma levels with VTE.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Blood
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Bruzelius, Maria
-
Iglesias, Maria ...
-
Hong, Mun-Gwan
-
Sanchez-Rivera, ...
-
Gyorgy, Beata
-
Carlos Souto, Ju ...
-
show more...
-
Frånberg, Mattia ...
-
Fredolini, Claud ...
-
Strawbridge, Ron ...
-
Holmström, Marga ...
-
Hamsten, Anders
-
Uhlén, Mathias
-
Silveira, Angela
-
Manuel Soria, Jo ...
-
Smadja, David M.
-
Butler, Lynn M.
-
Schwenk, Jochen ...
-
Morange, Pierre- ...
-
Tregouet, David- ...
-
Odeberg, Jacob
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Hematology
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Clinical Laborat ...
- Articles in the publication
-
Blood
- By the university
-
Stockholm University
-
Royal Institute of Technology
-
Karolinska Institutet