Cubosomes post-loaded with antimicrobial peptides: Characterization, bactericidal effect and proteolytic stability

Boge, Lukas, 1987 (författare): RISE,Chalmers University of Technology,Research Institutes of Sweden (RISE),Kemi och material

Umerska, Anita (författare): INSERM U 1066, France ; Université Angers, France,National Institute for Health and Medical Research, France

Matougui, Nada (författare): INSERM U 1066, France ; Université Angers,France,National Institute for Health and Medical Research, France

Bysell, Helena (författare): RISE,SP Sveriges Tekniska Forskningsinstitut AB,Research Institutes of Sweden (RISE),Kemi och material

Ringstad, Lovisa (författare): RISE,SP Sveriges Tekniska Forskningsinstitut AB,Research Institutes of Sweden (RISE),Kemi och material

Davoudi, Mina (författare): Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University, Sweden

Eriksson, Jonny (författare): Uppsala universitet,Uppsala University,Analytisk kemi,Edwards grupp,Uppsala University, Sweden

Edwards, Katarina (författare): Uppsala universitet,Uppsala University,Analytisk kemi,Edwards grupp,Uppsala University, Sweden

Andersson, Martin, 1974 (författare): Chalmers University of Technology,Chalmers University of Technology, Sweden

(creator_code:org_t)

Elsevier BV, 2017
2017
Engelska.
Ingår i: International Journal of Pharmaceutics. - : Elsevier BV. - 0378-5173 .- 1873-3476. ; 526:1-2, s. 400-412

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Abstract Ämnesord

Stäng

Novel antibiotics, such as antimicrobial peptides (AMPs), have recently attended more and more attraction. In this work, dispersed cubic liquid crystalline gel (cubosomes) was used as drug delivery vehicles for three AMPs (AP114, DPK-060 and LL-37). Association of peptides onto cubosomes was studied at two cubosome/peptide ratios using high performance liquid chromatography, ?-potential and circular dichroism measurements. AMPs impact on the cubosome structure was investigated using small angle x-ray scattering and cryogenic transmission electron microscopy. The antimicrobial effect of the AMP loaded cubosomes was studied in vitro by minimum inhibitory concentration and time-kill assays. Proteolytic protection was investigated by incubating the formulations with two elastases and the antimicrobial effect after proteolysis was studied using radial diffusion assay. Different association efficacy onto the cubosomes was observed among the AMPs, with LL-37 showing greatest association (>60%). AP114 loaded cubosomes displayed a preserved antimicrobial effect, whereas for LL-37 the broad spectrum bacterial killing was reduced to only comprise Gram-negative bacteria. Interestingly, DPK-060 loaded cubosomes showed a slight enhanced effect against S. aureus and E. coli strains. Moreover, the cubosomes were found to protect LL-37 from proteolytic degradation, resulting in a significantly better bactericidal effect after being subjected to elastase, compared to unformulated peptide.

NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Av författaren/redakt...: Boge, Lukas, 198 ...; Umerska, Anita; Matougui, Nada; Bysell, Helena; Ringstad, Lovisa; Davoudi, Mina; visa fler...; Eriksson, Jonny; Edwards, Katarin ...; Andersson, Marti ...; visa färre...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Farmakologi och ...

Av lärosätet: Chalmers tekniska högskola; Lunds universitet; Uppsala universitet; RISE

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