WFRF:(Palinauskas Vaidas)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 06951naa a2200457 4500 | |
| 001 | oai:lup.lub.lu.se:980dab26-f1cd-4ebb-9647-56bc23b52e87 | |
| 003 | SwePub | |
| 008 | 170315s2017 | |||||||||||000 ||eng| | |
| 009 | oai:DiVA.org:uu-517155 | |
| 024 | 7 | a https://lup.lub.lu.se/record/980dab26-f1cd-4ebb-9647-56bc23b52e872 URI |
| 024 | 7 | a https://doi.org/10.1111/mec.140852 DOI |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5171552 URI |
| 040 | a (SwePub)lud (SwePub)uu | |
| 041 | a engb engb swe | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Videvall, Elinu Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Molekylär ekologi och evolution,Forskargrupper vid Lunds universitet,Department of Biology,Faculty of Science,Molecular Ecology and Evolution Lab,Lund University Research Groups,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden4 aut0 (Swepub:uu)elivi343 |
| 245 | 1 0 | a The transcriptome of the avian malaria parasite Plasmodium ashfordi displays host-specific gene expression |
| 264 | c 2017-04-08 | |
| 264 | 1 | b Wiley,c 2017 |
| 520 | a Malaria parasites (Plasmodium spp.) include some of the world's most widespread and virulent pathogens. Our knowledge of the molecular mechanisms these parasites use to invade and exploit hosts other than mice and primates is, however, extremely limited. It is therefore imperative to characterize transcriptome-wide gene expression from non-model malaria parasites and how this varies across host individuals. Here, we used high-throughput Illumina RNA-sequencing on blood from wild-caught Eurasian siskins experimentally infected with a clonal strain of the avian malaria parasite Plasmodium ashfordi (lineage GRW2). By using a multi-step approach to filter out host transcripts, we successfully assembled the blood-stage transcriptome of P. ashfordi. A total of 11 954 expressed transcripts were identified, and 7 860 were annotated with protein information. We quantified gene expression levels of all parasite transcripts across three hosts during two infection stages – peak and decreasing parasitemia. Interestingly, parasites from the same host displayed remarkably similar expression profiles during different infection stages, but showed large differences across hosts, indicating that P. ashfordi may adjust its gene expression to specific host individuals. We further show that the majority of transcripts are most similar to the human parasite Plasmodium falciparum, and a large number of red blood cell invasion genes were discovered, suggesting evolutionary conserved invasion strategies between mammalian and avian Plasmodium. The transcriptome of P. ashfordi and its host-specific gene expression advances our understanding of Plasmodium plasticity and is a valuable resource as it allows for further studies analysing gene evolution and comparisons of parasite gene expression. | |
| 520 | a Malaria parasites (Plasmodium spp.) include some of the world's most widespread and virulent pathogens. Our knowledge of the molecular mechanisms these parasites use to invade and exploit hosts other than mice and primates is, however, extremely limited. It is therefore imperative to characterize transcriptome-wide gene expression from non-model malaria parasites and how this varies across host individuals. Here, we used high-throughput Illumina RNA-sequencing on blood from wild-caught Eurasian siskins experimentally infected with a clonal strain of the avian malaria parasite Plasmodium ashfordi (lineage GRW2). By using a multi-step approach to filter out host transcripts, we successfully assembled the blood-stage transcriptome of P. ashfordi. A total of 11 954 expressed transcripts were identified, and 7 860 were annotated with protein information. We quantified gene expression levels of all parasite transcripts across three hosts during two infection stages – peak and decreasing parasitemia. Interestingly, parasites from the same host displayed remarkably similar expression profiles during different infection stages, but showed large differences across hosts, indicating that P. ashfordi may adjust its gene expression to specific host individuals. We further show that the majority of transcripts are most similar to the human parasite Plasmodium falciparum, and a large number of red blood cell invasion genes were discovered, suggesting evolutionary conserved invasion strategies between mammalian and avian Plasmodium. The transcriptome of P. ashfordi and its host-specific gene expression advances our understanding of Plasmodium plasticity and is a valuable resource as it allows for further studies analysing gene evolution and comparisons of parasite gene expression. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Zoologi0 (SwePub)106082 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Zoology0 (SwePub)106082 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng |
| 653 | a RNA-seq | |
| 653 | a host-parasite interaction | |
| 700 | 1 | a Cornwallis, Charlieu Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden4 aut0 (Swepub:lu)biol-ccn |
| 700 | 1 | a Ahrén, Dagu Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden;National Bioinformatics Infrastructure Sweden (NBIS) Lund University Sölvegatan 37 SE‐22362 Lund Sweden4 aut0 (Swepub:lu)mbek-dah |
| 700 | 1 | a Palinauskas, Vaidasu State Scientific Institute Nature Research Centre,Institute of Ecology Nature Research Centre Akademijos 2 LT‐08412 Vilnius Lithuania4 aut |
| 700 | 1 | a Valkiunas, Gediminasu State Scientific Institute Nature Research Centre,Institute of Ecology Nature Research Centre Akademijos 2 LT‐08412 Vilnius Lithuania4 aut |
| 700 | 1 | a Hellgren, Olofu Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden4 aut0 (Swepub:lu)zooe-ohe |
| 710 | 2 | a MEMEGb Biologiska institutionen4 org |
| 773 | 0 | t Molecular Ecologyd : Wileyx 0962-1083x 1365-294X |
| 856 | 4 | u http://dx.doi.org/10.1111/mec.14085y FULLTEXT |
| 856 | 4 | u https://www.biorxiv.org/content/biorxiv/early/2017/02/28/072454.full.pdf |
| 856 | 4 8 | u https://lup.lub.lu.se/record/980dab26-f1cd-4ebb-9647-56bc23b52e87 |
| 856 | 4 8 | u https://doi.org/10.1111/mec.14085 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-517155 |
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