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Effect of ambient t...
Effect of ambient temperature on respiratory tract cells exposed to SARS-CoV-2 viral mimicking nanospheres - An experimental study
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- Paul, Alexandra, 1988 (författare)
- The University of Texas at Austin, USA,Chalmers tekniska högskola,Chalmers University of Technology
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- Kumar, Sachin (författare)
- The University of Texas at Austin, USA
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- Chatterjee, Sayantani (författare)
- Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG)
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- Pütz, Sabine (författare)
- Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG)
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- Nehra, Natasha (författare)
- The University of Texas at Austin, USA
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- Wang, Daniel S. (författare)
- The University of Texas at Austin, USA
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- Nisar, Arsalan (författare)
- The University of Texas at Austin, USA
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- Jennings, Christian M. (författare)
- The University of Texas at Austin, USA
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- Parekh, Sapun H. (författare)
- The University of Texas at Austin, USA,Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG)
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(creator_code:org_t)
- American Vacuum Society, 2021
- 2021
- Engelska.
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Ingår i: Biointerphases. - : American Vacuum Society. - 1559-4106 .- 1934-8630. ; 16:1
- Relaterad länk:
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https://www.ncbi.nlm...
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https://research.cha...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The novel coronavirus caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has reached more than 160 countries and has been declared a pandemic. SARS-CoV-2 infects host cells by binding to the angiotensin-converting enzyme 2 (ACE-2) surface receptor via the spike (S) receptor-binding protein (RBD) on the virus envelope. Global data on a similar infectious disease spread by SARS-CoV-1 in 2002 indicated improved stability of the virus at lower temperatures facilitating its high transmission in the community during colder months (December–February). Seasonal viral transmissions are strongly modulated by temperatures, which can impact viral trafficking into host cells; however, an experimental study of temperature-dependent activity of SARS-CoV-2 is still lacking. We mimicked SARS-CoV-2 with polymer beads coated with the SARS-CoV-2 S protein to study the effect of seasonal temperatures on the binding of virus-mimicking nanospheres to lung epithelia. The presence of the S protein RBD on nanosphere surfaces led to binding by Calu-3 airway epithelial cells via the ACE-2 receptor. Calu-3 and control fibroblast cells with S-RBD-coated nanospheres were incubated at 33 and 37 °C to mimic temperature fluctuations in the host respiratory tract, and we found no temperature dependence in contrast to nonspecific binding of bovine serum ablumin-coated nanospheres. Moreover, the ambient temperature changes from 4 to 40 °C had no effect on S-RBD-ACE-2 ligand-receptor binding and minimal effect on the S-RBD protein structure (up to 40 °C), though protein denaturing occurred at 51 °C. Our results suggest that ambient temperatures from 4 to 40 °C have little effect on the SARS-CoV-2-ACE-2 interaction in agreement with the infection data currently reported.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- protein structure
- materials properties
- epithelial cells
- viruses
- binding protein
- epithelium
- diseases and conditions
- nanomaterials
- enzymes
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Paul, Alexandra, ...
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Kumar, Sachin
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Chatterjee, Saya ...
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Pütz, Sabine
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Nehra, Natasha
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Wang, Daniel S.
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visa fler...
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Nisar, Arsalan
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Jennings, Christ ...
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Parekh, Sapun H.
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Andra medicinska ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Mikrobiologi ino ...
- Artiklar i publikationen
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Biointerphases
- Av lärosätet
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Chalmers tekniska högskola