A modified culture-based study of bacterial community composition in a tannery wastewater treatment plant

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Fältnamn	Indikatorer	Metadata
000		03385naa a2200397 4500
001		oai:DiVA.org:kth-27651
003		SwePub
008		101220s2010 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-276512 URI
024	7	a https://doi.org/10.2166/wst.2010.5482 DOI
040		a (SwePub)kth
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Desta, Adey F.u KTH,Miljömikrobiologi4 aut0 (Swepub:kth)u1s0br13
245	1 0	a A modified culture-based study of bacterial community composition in a tannery wastewater treatment plant
264	1	b IWA Publishing,c 2010
338		a print2 rdacarrier
500		a QC 20101223
520		a Though culture-independent methods have been used in preference to traditional isolation techniques for characterization of microbial community of wastewater treatment plants, it is difficult to widely apply this approach in resource-poor countries. The present study aimed to develop a test to identify the culturable portion of bacterial community in a high-strength wastewater. Wastewater samples were collected from nitrification-denitrification and settling tanks of the treatment plant of Elmo Leather AB tannery located in Boras, Sweden. After cultivating on nutrient agar with the optimal dilution (10(-2)), phenotypic and biochemical identification of the bacteria were done with colony morphology, Gram reaction, growth on MacConkey, phenylethanol media, triple sugar Iron agar slants, catalase and oxidase tests. Biochemical grouping of the isolates was done based on their test results for MacConkey, phenylethanol media, triple sugar Iron agar and oxidase test reaction. From the biochemical groups, isolates were randomly selected for API test and 16SrRNA gene sequencing. The isolates from the denitrification, nitrification tank were identified to be Paracoccus denitrificans (67%), Azoarcus spp (3%) and Spingomonas wittichii (1%). From the settling tank, Paracoccus denitrificans (22%), Corynebacterium freneyi (20%) and Bacillus cereus (1%) were identified. The grouping based on biochemical test results as well as the identification based on sequencing has shown coherence except for discrepancies with the API test. The preliminary implications of the grouping based on culture-based characteristics and its potential application for resource-limited environmental microbial studies is discussed.
650	7	a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653		a biochemical grouping
653		a culture-dependent
653		a microbial community
653		a tannery wastewater
653		a triple sugar iron agar
653		a Biochemistry
653		a Biokemi
700	1	a Dalhammer, Gunnelu KTH,Miljömikrobiologi4 aut0 (Swepub:kth)u1yfiunx
700	1	a Rajarao, Gunaratna Kuttuvau KTH,Miljömikrobiologi4 aut0 (Swepub:kth)u12ci1fh
710	2	a KTHb Miljömikrobiologi4 org
773	0	t Water Science and Technologyd : IWA Publishingg 62:11, s. 2543-2549q 62:11<2543-2549x 0273-1223x 1996-9732
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-27651
856	4 8	u https://doi.org/10.2166/wst.2010.548

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Av författaren/redakt...: Desta, Adey F.; Dalhammer, Gunne ...; Rajarao, Gunarat ...

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Biokemi och mole ...

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