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PHA-induced IL-12Rb...
PHA-induced IL-12Rb2 mRNA expression in atopic and non-atopic children
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- Janefjord, Camilla (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Jenmalm, Maria (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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(creator_code:org_t)
- 2008-07-07
- 2001
- Engelska.
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Ingår i: Clinical and Experimental Allergy. - : Wiley. - 0954-7894. ; 31:10, s. 1493-1500
- Relaterad länk:
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http://urn.kb.se/res...
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visa fler...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background IL-12 is a strong inducer of Th1 responses. Stimulation via the CD2 receptor increases IFN-γ production and enhances the responsiveness of activated T-cells to IL-12, possibly due to an up-regulation of the signal transducing β2 chain of the IL-12 receptor (IL-12Rβ2). Atopic children have a reduced Th1-like immunity and a reduced CD2 expression. Our hypothesis is that atopic individuals have a reduced function of the CD2 pathway, causing reduced responsiveness to IL-12 and decreased IFN-γ production. Objective The aim was to study the mRNA expression of the IL-12Rβ2 chain, after stimulation via the CD2 pathway in peripheral blood mononuclear cells (PBMC), of atopic and non-atopic children, and to investigate correlations to the production of Th1 and Th2 cytokines. Materials and methods The study included 23 skin prick test positive, and 9 non-sensitized, 12-year-old children. PBMC were stimulated for 24 h with phytohemagglutinin (PHA) (2 µg/mL), which stimulates T cells through the CD2 pathway. Expression of IL-12Rβ2 mRNA was analysed by quantitative real time PCR and the cytokine production was detected with ELISA. Results Atopic and non-atopic children had similar baseline expression of IL-12Rβ2 mRNA, whereas PHA-induced IL-12Rβ2 mRNA expression was lower in atopic than in non-atopic children. The PHA-induced IL-12Rβ2 mRNA expression correlated well with the PHA-induced IFN-γ production and with the IFN-γ/IL-4 ratio. Conclusion PBMC from atopic children expressed less IL-12Rβ2 mRNA than non-atopic children after stimulation via the CD2 pathway (PHA). This may indicate a reduced capacity to respond to Th1-inducing stimuli in atopic children.
Nyckelord
- CD2
- PHA
- IL-12Rβ2
- Quantitative Real Time PCR
- ELISA
- cytokine
- atopic children
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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