Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor: Complementary Match of Supramolecular and Dynamic Chemistry

Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor : Complementary Match of Supramolecular and Dynamic Chemistry

Bergdahl, Gizem Ertürk (author): Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),CapSenze Biosystems AB, Lund, Sweden

Akhoundian, Maedeh (author): Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV)

Lueg-Althoff, Kyra (author): Department of Chemistry, University of Duisburg-Essen, Universitätsstr. 7, Essen, 45117, Germany

Shinde, Sudhirkumar (author): Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,School of Chemistry and Chemical Engineering, Queens University Belfast, Northern Ireland, United Kingdom

Yeung, Sing Yee (author): Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces

Hedström, Martin (author): CapSenze Biosystems AB, Lund, 223 63, Sweden; Department of Biotechnology, Lund University, Lund, 223 62, Sweden

Schrader, Thomas (author): Department of Chemistry, University of Duisburg-Essen, Universitätsstr. 7, Essen, 45117, Germany

Mattiasson, Bo (author): CapSenze Biosystems AB, Lund, 223 63, Sweden; Department of Biotechnology, Lund University, Lund, 223 62, Sweden

Sellergren, Börje (author): Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces

(creator_code:org_t)

Royal Society of Chemistry, 2019
2019
English.
In: New Journal of Chemistry. - : Royal Society of Chemistry. - 1144-0546 .- 1369-9261. ; 43:2, s. 847-852

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for Trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the strong interaction of bisphosphonate with lysine/arginine residues on the surface of Trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.

By the author/editor: Bergdahl, Gizem ...; Akhoundian, Maed ...; Lueg-Althoff, Ky ...; Shinde, Sudhirku ...; Yeung, Sing Yee; Hedström, Martin; show more...; Schrader, Thomas; Mattiasson, Bo; Sellergren, Börj ...; show less...

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