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Sökning: WFRF:(Jin Hongfang) > (2014) > L-Cystathionine Inh...

L-Cystathionine Inhibits the Mitochondria-Mediated Macrophage Apoptosis Induced by Oxidized Low Density Lipoprotein

Zhu, Mingzhu (författare)
Peking University, Peoples R China
Du, Junbao (författare)
Peking University, Peoples R China; Minist Educ, Peoples R China
Chen, Siyao (författare)
Peking University, Peoples R China
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Dong Liu, Angie (författare)
Holmberg, Lukas (författare)
Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
Chen, Yonghong (författare)
Peking University, Peoples R China
Zhang, Chunyu (författare)
Peking University, Peoples R China
Tang, Chaoshu (författare)
Minist Educ, Peoples R China; Peking University, Peoples R China
Jin, Hongfang (författare)
Peking University, Peoples R China
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 (creator_code:org_t)
2014-12-11
2014
Engelska.
Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 15:12, s. 23059-23073
Relaterad länk:
https://liu.diva-por... (primary) (Raw object)
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https://www.mdpi.com...
https://urn.kb.se/re...
https://doi.org/10.3...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • This study was designed to investigate the regulatory role of L-cystathionine in human macrophage apoptosis induced by oxidized low density lipoprotein (ox-LDL) and its possible mechanisms. THP-1 cells were induced with phorbol 12-myristate 13-acetate (PMA) and differentiated into macrophages. Macrophages were incubated with ox-LDL after pretreatment with L-cystathionine. Superoxide anion, apoptosis, mitochondrial membrane potential, and mitochondrial permeability transition pore (MPTP) opening were examined. Caspase-9 activities and expression of cleaved caspase-3 were measured. The results showed that compared with control group, ox-LDL treatment significantly promoted superoxide anion generation, release of cytochrome c (cytc) from mitochondrion into cytoplasm, caspase-9 activities, cleavage of caspase-3, and cell apoptosis, in addition to reduced mitochondrial membrane potential as well as increased MPTP opening. However, 0.3 and 1.0 mmol/L L-cystathionine significantly reduced superoxide anion generation, increased mitochondrial membrane potential, and markedly decreased MPTP opening in ox-LDL + L-cystathionine macrophages. Moreover, compared to ox-LDL treated-cells, release of cytc from mitochondrion into cytoplasm, caspase-9 activities, cleavage of caspase-3, and apoptosis levels in L-cystathionine pretreated cells were profoundly attenuated. Taken together, our results suggested that L-cystathionine could antagonize mitochondria-mediated human macrophage apoptosis induced by ox-LDL via inhibition of cytc release and caspase activation.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine (hsv//eng)

Nyckelord

L-cystathionine; ox-LDL; macrophage; mitochondrial; oxidative stress; apoptosis; caspase-9; caspase-3

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