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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004578naa a2200601 4500
001oai:DiVA.org:liu-95509
003SwePub
008130705s2013 | |||||||||||000 ||eng|
009oai:slubar.slu.se:40723
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-955092 URI
0247 a https://doi.org/10.1016/j.anireprosci.2013.02.0212 DOI
0247 a https://res.slu.se/id/publ/407232 URI
040 a (SwePub)liud (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Balao da Silva, C M.u University of Extremadura Caceres, Spain4 aut
2451 0a Sex sorting increases the permeability of the membrane of stallion spermatozoa
264 1b Elsevier Masson,c 2013
338 a print2 rdacarrier
500 a Funding Agencies|Ministerio de Ciencia e Innovacion-FEDER in Madrid, Spain|AGL 2010-20758BFU2011-30261|Junta de Extremadura, Merida, Spain-FEDER|10010PCE1002|
520 a At present, the only repeatable means of selecting the sex of offspring is the Beltsville semen sorting technology using flow cytometry (FC). This technology has reached commercial status in the bovine industry and substantial advances have occurred recently in swine and ovine species. In the equine species, however, the technology is not as well developed. To better understand the changes induced in stallion spermatozoa during the sorting procedure, pooled sperm samples were sorted: sperm motility and kinematics were assessed using computer assisted sperm analysis, sperm membrane integrity was assessed using the YoPro-1 assay, while plasmalemmal stability and lipid architecture were assessed using Merocyanine 540/SYTOX green and Annexin-V, respectively. Lipid peroxidation was also investigated with the probe Bodipy(581/591)-C11. All assays were performed shortly after collection, after incubation and after sex sorting using FC. In order to characterize potential molecular mechanisms implicated in sperm damage, an apoptosis protein antibody dot plot array analysis was performed before and after sorting. While the percentage of total motile sperm remained unchanged, sex sorting reduced the percentages of progressive motile spermatozoa and of rapid spermatozoa as well as curvilinear velocity (VCL). Sperm membranes responded to sorting with an increase in the percentage of YoPro-1 positive cells, suggesting the sorted spermatozoa had a reduced energy status that was confirmed by measuring intracellular ATP content.
650 7a LANTBRUKSVETENSKAPERx Veterinärmedicinx Annan veterinärmedicin0 (SwePub)403042 hsv//swe
650 7a AGRICULTURAL SCIENCESx Veterinary Sciencex Other Veterinary Science0 (SwePub)403042 hsv//eng
653 a Stallion
653 a Apoptosis
653 a Protein antibody array
653 a Sex sorting
653 a Flow cytometry
653 a Ca2+
653 a Fluo 3
653 a YoPro-1
653 a ATP
653 a Annexin-V
653 a Merocyanine 540
653 a TECHNOLOGY
653 a TEKNIKVETENSKAP
7001 a Ortega Ferrusola, Cu University of Extremadura Caceres, Spain4 aut
7001 a Morillo Rodriguez, Au University of Extremadura Caceres, Spain4 aut
7001 a Morrell, Janeu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,University of Extremadura Caceres, Spain,Swedish University of Agriculture Science, Sweden4 aut0 (Swepub:slu)50646
7001 a Plaza Davila, Mu University of Extremadura Caceres, Spain4 aut
7001 a Morrell, J M.u Swedish University of Agriculture Science, Sweden4 aut
7001 a Rodriguez Martinez, Hu Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Hälsouniversitetet4 aut0 (Swepub:liu)herro55
7001 a Tapia, J A.u University of Extremadura Caceres, Spain4 aut
7001 a Aparicio, I M.u University of Extremadura Caceres, Spain4 aut
7001 a Pena, F -j.u University of Extremadura Caceres, Spain4 aut
7102 a University of Extremadura Caceres, Spainb Institutionen för kliniska vetenskaper (KV)4 org
7102 a Sveriges lantbruksuniversitet
7730 t Animal Reproduction Scienced : Elsevier Massong 138:3-4, s. 241-251q 138:3-4<241-251x 0378-4320x 1873-2232
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-95509
8564 8u https://doi.org/10.1016/j.anireprosci.2013.02.021
8564 8u https://res.slu.se/id/publ/40723

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