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Fluorescent nucleob...
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Bood, MattiasGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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Fluorescent nucleobase analogues for base-base FRET in nucleic acids: Synthesis, photophysics and applications
- Article/chapterEnglish2018
Publisher, publication year, extent ...
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2018-01-10
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Beilstein Institut,2018
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/264212
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https://gup.ub.gu.se/publication/264212URI
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https://doi.org/10.3762/bjoc.14.7DOI
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https://research.chalmers.se/publication/500458URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Forster resonance energy transfer (FRET) between a donor nucleobase analogue and an acceptor nucleobase analogue, base-base FRET, works as a spectroscopic ruler and protractor. With their firm stacking and ability to replace the natural nucleic acid bases inside the base-stack, base analogue donor and acceptor molecules complement external fluorophores like the Cy-, Alexa- and ATTO-dyes and enable detailed investigations of structure and dynamics of nucleic acid containing systems. The first base-base FRET pair, tCO-tCnitro, has recently been complemented with among others the adenine analogue FRET pair, qAN1-qAnitro, increasing the flexibility of the methodology. Here we present the design, synthesis, photophysical characterization and use of such base analogues. They enable a higher control of the FRET orientation factor, κ2, have a different distance window of opportunity than external fluorophores, and, thus, have the potential to facilitate better structure resolution. Netropsin DNA binding and the B-to-Z-DNA transition are examples of structure investigations that recently have been performed using base.base FRET and that are described here. Base-base FRET has been around for less than a decade, only in 2017 expanded beyond one FRET pair, and represents a highly promising structure and dynamics methodology for the field of nucleic acids. Here we bring up its advantages as well as disadvantages and touch upon potential future applications. © 2018 Bood et al.
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Sarangamath, Sangamesh,1992Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)sansar
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Sandberg Wranne, Moa,1986Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)moasan
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Grøtli, Morten,1966Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)grotli
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Wilhelmsson, Marcus,1974Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mawi
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Göteborgs universitetInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
Related titles
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In:Beilstein Journal of Organic Chemistry: Beilstein Institut14, s. 114-1291860-5397
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