Congenital tremor and splay leg in piglets - insights into the virome, local cytokine response, and histology

Stenberg, HedvigSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health (författare)

Background: Atypical porcine pestivirus (APPV) is a neurotropic virus associated with congenital tremor type A-II. A few experimental studies also indicate an association between APPV and splay leg. The overarching aim of the present study was to provide insights into the virome, local cytokine response, and histology of the CNS in piglets with signs of congenital tremor or splay leg.Results: Characterization of the cytokine profile and virome of the brain in piglets with signs of congenital tremor revealed an APPV-associated upregulation of Stimulator of interferon genes (STING). The upregulation of STING was associated with an increased expression of the gene encoding IFN-alpha but no differential expression was recorded for the genes encoding CXCL8, IFN-beta, IFN-gamma, IL-1 beta, IL-6, or IL-10. No viral agents or cytokine upregulation could be detected in the spinal cord of piglets with signs of splay leg or in the brain of piglets without an APPV-infection. The histopathological examination showed no lesions in the CNS that could be attributed to the APPV-infection, as no difference between sick and healthy piglets could be seen.Conclusion: The results from this study provide evidence of an APPV-induced antiviral cytokine response but found no lesions related to the infection nor any support for a common causative agent.

Hellman, StinaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health (författare)
Lindström, LisaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)97492 (författare)
Jacobson, MagdalenaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences(Swepub:slu)49988 (författare)
Fossum, CarolineSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)47429 (författare)
Malmberg, MajaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Institutionen för husdjursgenetik (HGEN),Department of Biomedical Science and Veterinary Public Health,Department of Animal Breeding and Genetics(Swepub:slu)97433 (författare)
Sveriges lantbruksuniversitetInst för biomedicin och veterinär folkhälsovetenskap (creator_code:org_t)
Sveriges lantbruksuniversitet

Ingår i:BMC Veterinary Research: Springer Science and Business Media LLC181746-6148

Av författaren/redakt...: Stenberg, Hedvig; Hellman, Stina; Lindström, Lisa; Jacobson, Magdal ...; Fossum, Caroline; Malmberg, Maja

Om ämnet

LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Veterinärmedicin; och Patobiologi

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