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Sökning: WFRF:(Sundberg Olle) > (2020-2024) > Multiplexing DNA me...

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FältnamnIndikatorerMetadata
00006189naa a2200601 4500
001oai:DiVA.org:uu-423036
003SwePub
008201021s2020 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4230362 URI
0247 a https://doi.org/10.1172/jci.insight.1365792 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Neiman, Danielu Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
2451 0a Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic β cells
264 c 2020-07-23
264 1b American Society for Clinical Investigation,c 2020
338 a print2 rdacarrier
500 a Wos title: Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic beta cells
520 a It has been proposed that unmethylated insulin promoter fragments in plasma derive exclusively from β cells, reflect their recent demise, and can be used to assess β cell damage in type 1 diabetes. Herein we describe an ultrasensitive assay for detection of a β cell–specific DNA methylation signature, by simultaneous assessment of 6 DNA methylation markers, that identifies β cell DNA in mixtures containing as little as 0.03% β cell DNA (less than 1 β cell genome equivalent). Based on this assay, plasma from nondiabetic individuals (N = 218, aged 4–78 years) contained on average only 1 β cell genome equivalent/mL. As expected, cell-free DNA (cfDNA) from β cells was significantly elevated in islet transplant recipients shortly after transplantation. We also detected β cell cfDNA in a patient with KATP congenital hyperinsulinism, in which substantial β cell turnover is thought to occur. Strikingly, in contrast to previous reports, we observed no elevation of β cell–derived cfDNA in autoantibody-positive subjects at risk for type 1 diabetes (N = 32), individuals with recent-onset type 1 diabetes (<4 months, N = 92), or those with long-standing disease (>4 months, N = 38). We discuss the utility of sensitive β cell cfDNA analysis and potential explanations for the lack of a β cell cfDNA signal in type 1 diabetes.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng
7001 a Gillis, Davidu Hadassah Hebrew Univ, Endocrine Unit, Dept Pediat, Med Ctr, Jerusalem, Israel.4 aut
7001 a Piyanzin, Sheinau Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
7001 a Cohen, Danielu Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
7001 a Fridlich, Oriu Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
7001 a Moss, Joshuau Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
7001 a Zick, Aviadu Hadassah Hebrew Univ, Sharett Inst Oncol, Med Ctr, Jerusalem, Israel.4 aut
7001 a Oron, Talu Schneider Childrens Med Ctr Israel, Inst Endocrinol & Diabet, Petah Tiqwa, Israel.4 aut
7001 a Sundberg, Fridau Sahlgrens Univ Hosp, Queen Silvia Childrens Hosp, Gothenburg, Sweden.4 aut
7001 a Forsander, Gunu Sahlgrens Univ Hosp, Queen Silvia Childrens Hosp, Gothenburg, Sweden.4 aut
7001 a Skog, Oskar,c Docent, PhD,d 1981-u Uppsala universitet,Klinisk immunologi4 aut0 (Swepub:uu)ossko535
7001 a Korsgren, Olleu Uppsala universitet,Klinisk immunologi4 aut0 (Swepub:uu)ollekors
7001 a Levy-Khademi, Florisu Shaare Zedek Med Ctr, Dept Pediat, Jerusalem, Israel.4 aut
7001 a Arbel, Danu Hadassah Hebrew Univ, Med Ctr, Pediat Surg, Jerusalem, Israel.4 aut
7001 a Hashavia, Saaru Hadassah Hebrew Univ, Med Ctr, Pediat Emergency, Jerusalem, Israel.4 aut
7001 a Shapiro, A. M. Jamesu Univ Alberta, Li Ka Shing Ctr Hlth Res Innovat, Edmonton, AB, Canada.4 aut
7001 a Speake, Cateu Benaroya Res Inst, Diabet Program, Seattle, WA USA.4 aut
7001 a Greenbaum, Carlau Benaroya Res Inst, Diabet Program, Seattle, WA USA.4 aut
7001 a Hosford, Jenniferu Univ Florida, Diabet Inst, Dept Pediat, Gainesville, FL 32610 USA.;Univ Florida, Diabet Inst, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA.4 aut
7001 a Posgai, Amandau Univ Florida, Coll Med, Gainesville, FL 32610 USA.4 aut
7001 a Atkinson, Mark A.u Univ Florida, Diabet Inst, Dept Pediat, Gainesville, FL 32610 USA.;Univ Florida, Diabet Inst, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA.;Univ Florida, Coll Med, Gainesville, FL 32610 USA.4 aut
7001 a Glaser, Benjaminu Hadassah Hebrew Univ, Dept Internal Med, Endocrinol & Metab Serv, Med Ctr, Jerusalem, Israel.4 aut
7001 a Schatz, Desmond A.u Univ Florida, Diabet Inst, Dept Pediat, Gainesville, FL 32610 USA.;Univ Florida, Diabet Inst, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA.4 aut
7001 a Shemer, Ruthu Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
7001 a Dor, Yuvalu Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.4 aut
7102 a Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.b Hadassah Hebrew Univ, Endocrine Unit, Dept Pediat, Med Ctr, Jerusalem, Israel.4 org
7730 t JCI Insightd : American Society for Clinical Investigationg 5:14q 5:14x 2379-3708
8564 u http://insight.jci.org/articles/view/136579/files/pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-423036
8564 8u https://doi.org/10.1172/jci.insight.136579

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