WFRF:(Von Stedingk Henrik)
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03698naa a2200361 4500 | |
| 001 | oai:DiVA.org:su-112092 | |
| 003 | SwePub | |
| 008 | 150109s2014 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1120922 URI |
| 024 | 7 | a https://doi.org/10.1021/tx50027492 DOI |
| 040 | a (SwePub)su | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Carlsson, Henriku Stockholms universitet,Institutionen för material- och miljökemi (MMK)4 aut0 (Swepub:su)hecar |
| 245 | 1 0 | a LC–MS/MS Screening Strategy for Unknown Adducts to N-Terminal Valine in Hemoglobin Applied to Smokers and Nonsmokers |
| 264 | c 2014-11-06 | |
| 264 | 1 | b American Chemical Society (ACS),c 2014 |
| 338 | a print2 rdacarrier | |
| 520 | a Electrophilically reactive compounds have the ability to form adducts with nucleophilic sites in DNA and proteins, constituting a risk for toxic effects. Mass spectrometric detection of adducts to N-terminal valine in hemoglobin (Hb) after detachment by modified Edman degradation procedures is one approach for in vivo monitoring of exposure to electrophilic compounds/metabolites. So far, applications have been limited to one or a few selected reactive species, such as acrylamide and its metabolite glycidamide. This article presents a novel screening strategy for unknown Hb adducts to be used as a basis for an adductomic approach. The method is based on a modified Edman procedure, FIRE, specifically developed for LC-MS/MS analysis of N-terminal valine adducts in Hb detached as fluorescein thiohydantoin (FTH) derivatives. The aim is to detect and identify a priori unknown Hb adducts in human blood samples. Screening of valine adducts was performed by stepwise scanning of precursor ions in small mass increments, monitoring four fragments common for the FTH derivative of valine with different N-substitutions in the multiple-reaction mode, covering a mass range of 135 Da (m/z 503-638). Samples from six smokers and six nonsmokers were analyzed. Control experiments were performed to compare these results with known adducts and to check for artifactual formation of adducts. In all samples of smokers and nonsmokers, seven adducts were identified, of which six have previously been studied. Nineteen unknown adducts were observed, and 14 of those exhibited fragmentation patterns similar to earlier studied FTH derivatives of adducts to valine. Identification of the unknown adducts will be the focus of future work. The presented methodology is a promising screening tool using Hb adducts to indicate exposure to potentially toxic electrophilic compounds and metabolites. | |
| 650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
| 653 | a Environmental Chemistry | |
| 653 | a miljökemi | |
| 700 | 1 | a von Stedingk, Hansu Stockholms universitet,Institutionen för material- och miljökemi (MMK)4 aut0 (Swepub:su)hvons |
| 700 | 1 | a Nilsson, Ulrikau Stockholms universitet,Institutionen för analytisk kemi4 aut0 (Swepub:su)unils |
| 700 | 1 | a Törnqvist, Margaretau Stockholms universitet,Institutionen för material- och miljökemi (MMK)4 aut0 (Swepub:su)mt |
| 710 | 2 | a Stockholms universitetb Institutionen för material- och miljökemi (MMK)4 org |
| 773 | 0 | t Chemical Research in Toxicologyd : American Chemical Society (ACS)g 27:12, s. 2062-2070q 27:12<2062-2070x 0893-228Xx 1520-5010 |
| 856 | 4 | u https://doi.org/10.1021/tx5002749 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-112092 |
| 856 | 4 8 | u https://doi.org/10.1021/tx5002749 |
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