Fine-tuning the stress response of Saccharomyces cerevisiae using CRISPR interference technology

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: AMNE:(ENGINEERING AND TECHNOLOGY Industrial Biotechnology Biochemicals) > (2015-2019) > Fine-tuning the str...

Fine-tuning the stress response of Saccharomyces cerevisiae using CRISPR interference technology

Mukherjee, Vaskar, 1986 (author): Chalmers tekniska högskola,Chalmers University of Technology

Cámara, Elena, 1985 (author): Chalmers tekniska högskola,Chalmers University of Technology

Trollmann, Ignatius, 1992 (author): Chalmers tekniska högskola,Chalmers University of Technology

Olsson, Lisbeth, 1963 (author): Chalmers tekniska högskola,Chalmers University of Technology

Nygård, Yvonne, 1986 (author): Chalmers tekniska högskola,Chalmers University of Technology

show less...

(creator_code:org_t)

2018
2018
English.

Related links:: http://conferences.g...; show more...; https://research.cha...; show less...

Conference paper (other academic/artistic)

Abstract Subject headings

Efficient biochemical conversion of renewable carbon sources is crucial for the transition into an entirely renewable energy system and a resource-efficient society. However, the substitution of fossil based biochemical with its renewable counterpart requires the production to be significantly more efficient and price competitive. Production of second-generation biochemicals (made from lignocellulosic biomass) is challenging due to presence of inhibitors in lignocellulose hydrolysate. Weak acids, furans and phenolic compounds that are formed or released during hydrolysis of biomass are toxic for the producing cells and leads to suboptimal yield and productivity obtained during fermentation. Numerous attempts have been reported to improve the stress tolerance of Saccharomyces cerevisiae by different bioengineering strategies such as deletion/overexpression of genes. However, the inability to achieve a fine balance of the transcriptional expression of the target and the ancillary gene(s) is one of the major factors that impedes the efficiency of many of these strategies. In this project, we apply CRISPR interference (CRISPRi) technology to investigate the potential of fine-tuning the expression of genes that are related to the stress regulation. CRISPRi is a genetic perturbation technique that allows sequence-specific repression or activation of gene expression, achieved by a catalytically inactive Cas9 protein fused to a repressor or activator, which can be targeted to any genetic loci using a sgRNA. Strains with altered regulation will be screened for inhibitor tolerance. Furthermore, transcriptomics analysis of tolerant mutants will be conducted to link superior phenotypes to the transcriptomic landscape. Subsequently, this novel information will be used as a resource to accelerate the design-build-test-learn cycle used for developing industrial yeast strains for efficient conversion of lignocellulosic hydrolysate. Here, we will show data on a methodology that we have developed for studying hydrolysate tolerance, adaptation and ethanol production capacity at microscale, directly in lignocellulosic hydrolysates.

Subject headings

TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
TEKNIK OCH TEKNOLOGIER -- Naturresursteknik -- Energisystem (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Environmental Engineering -- Energy Systems (hsv//eng)
NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)

Keyword

Transcription
CRISPRi
CRISPRa
Yeast
Stress tolerance
renewable energy
Second-generation biochemicals
CRISPR-Cas9

Publication and Content Type

kon (subject category)
vet (subject category)

To the university's database

Find more in SwePub

By the author/editor: Mukherjee, Vaska ...; Cámara, Elena, 1 ...; Trollmann, Ignat ...; Olsson, Lisbeth, ...; Nygård, Yvonne, ...

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Environmental En ...; and Energy Systems

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Bioinformatics a ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Genetics

By the university: Chalmers University of Technology

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se