Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

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Fältnamn	Indikatorer	Metadata
000		02460naa a2200313 4500
001		oai:DiVA.org:ri-27387
003		SwePub
008		161208s2003 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-273872 URI
040		a (SwePub)ri
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Allen, LT4 aut
245	1 0	a Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect
264	1	c 2003
338		a print2 rdacarrier
500		a A1622
520		a The assessment of biomaterial compatibility relies heavily on the analysis of macroscopic cellular responses to material interaction. However, new technologies have become available that permit a more profound understanding of the molecular basis of cell-biomaterial interaction. Here, both conventional phenotypic and contemporary transcriptomic (DNA microarray-based) analysis techniques were combined to examine the interaction of cells with a homologous series of copolymer films that subtly vary in terms of surface hydrophobicity. More specifically, we used differing combinations of N-isopropylacrylamide, which is presently used as an adaptive cell culture substrate, and the more hydrophobic, yet structurally similar, monomer N-tert-butylacrylamide. We show here that even discrete modifications with respect to the physiochemistry of soft amorphous materials can lead to significant impacts on the phenotype of interacting cells. Furthermore, we have elucidated putative links between phenotypic responses to cell-biomaterial interaction and global gene expression profile alterations. This case study indicates that high-throughput analysis of gene expression not only can greatly refine our knowledge of cell-biomaterial interaction, but also can yield novel biomarkers for potential use in biocompatibility assessment
700	1	a Fox, EJP4 aut
700	1	a Blute, Iu RISE,YKI – Ytkemiska institutet4 aut
700	1	a Kelly, ZD4 aut
700	1	a Rochev, Y4 aut
700	1	a Keenan, AK4 aut
700	1	a Dawson,4 aut
710	2	a RISEb YKI – Ytkemiska institutet4 org
773	0	t Proceedings of the National Academy of Sciences of the United States of Americag 100, s. 6331-6336q 100<6331-6336x 0027-8424x 1091-6490
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-27387

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Av författaren/redakt...: Allen, LT; Fox, EJP; Blute, I; Kelly, ZD; Rochev, Y; Keenan, AK; visa fler...; Dawson,; visa färre...

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