L773:0901 9928
Search: L773:0901 9928 > Spontaneous Reversa...
- 31 of 79
- Previous record
- Next record
- To hitlist
Spontaneous Reversal of P-Glycoprotein Expression in Multidrug Resistant Cell Lines
-
- Green, Henrik (author)
- Linköpings universitet,Hälsouniversitetet,Klinisk farmakologi
-
- Lotfi, Kourosh (author)
- Linköpings universitet,Hälsouniversitetet,Klinisk farmakologi
-
- Zackrisson, Anna Lena (author)
- Linköpings universitet,Hälsouniversitetet,Klinisk farmakologi
- show more...
- show less...
- (creator_code:org_t)
- Wiley, 2003
- 2003
- English.
- In: Pharmacology and Toxicology. - : Wiley. - 0901-9928 .- 1600-0773. ; 93:6, s. 297-304
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Increased expression of P-glycoprotein encoded by the mdr-1 gene is a well-characterised mechanism for resistance to cancer chemotherapeutic drugs in cell lines. However, the P-glycoprotein expression after removal of the selection pressure has not fully been elucidated. The stability of P-glycoprotein expression in the presence (+) and absence (-) of vincristine (30 or 150 nM) was studied in multidrug resistant K562 cell lines (VCR30+, VCR150+, VCR30- and VCR150-) for 11 months. The P-glycoprotein protein and mdr-1 mRNA levels were determined at regular intervals using flow cytometry and real-time PCR, respectively. Chemosensitivity to a panel of antineoplastic drugs was measured using an MTT assay. The presence of vincristine (VCR30+ and VCR150+) resulted in high and stable levels of P-glycoprotein and mdr-1 mRNA during the whole period compared to wild type. As for the VCR30- and VCR150- subcultures, the expressions of P-glycoprotein and mdr-1 mRNA were stable for five months, and then the levels decreased rapidly. Concomitantly, the sensitivity to drugs known as P-glycoprotein substrates was restored. In conclusion, resistant cells growing in the presence of the inducing drug have a stable P-glycoprotein expression and resistance level, but removing the inducing drug may result in a sudden and rapid lowering of P-glycoprotein and mdr-1 mRNA levels as long as five months after drug withdrawal.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Pharmacology and Toxicology (Search for host publication in LIBRIS)
To the university's database
- 31 of 79
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Green, Henrik
- Lotfi, Kourosh
- Zackrisson, Anna ...
- Peterson, Curt
- Articles in the publication
- Pharmacology and ...
- By the university
- Linköping University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
