Expression of claudin 1, claudin 4, and claudin 7 in colorectal cancer and its relation with CLDN DNA methylation patterns

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Fältnamn	Indikatorer	Metadata
000		05107naa a2200529 4500
001		oai:DiVA.org:oru-57335
003		SwePub
008		170530s2017 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:umu-136210
009		oai:DiVA.org:uu-323664
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-573352 URI
024	7	a https://doi.org/10.1177/10104283176975692 DOI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1362102 URI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3236642 URI
040		a (SwePub)orud (SwePub)umud (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Hahn-Strömberg, Victoriau Uppsala universitet,Institutionen för medicinsk cellbiologi4 aut0 (Swepub:uu)vicst533
245	1 0	a Expression of claudin 1, claudin 4, and claudin 7 in colorectal cancer and its relation with CLDN DNA methylation patterns
264		c 2017-04-05
264	1	b SAGE Open,c 2017
338		a print2 rdacarrier
500		a Funding agencies:Örebro Läns Landstings Forskningskommitte, Lions cancerfond, Uppsala-ÖrebroNyckelfonden, Örebro
520		a Altered claudin expression has been described in colon, prostatic, ovarian, and breast carcinoma. However, the role of epigenetic modifications in these genes and their role in colorectal cancer is unknown. We aimed our study to investigate whether claudin protein expression and methylation of CLDN can influence the tumorigenesis of colorectal cancer. A total of 31 patients diagnosed with colorectal carcinoma was used in this study. Immunohistochemical staining was used to study protein expression in both tumor and the adjacent nonneoplastic mucosa of claudin 1, 4, and 7. To detect the DNA methylation pattern of CLDN1, 4, and 7, genomic DNA was extracted from both the tumor and the adjacent nonneoplastic mucosa. Methylation analysis was carried out using bisulfite pyrosequencing. Cell membrane staining intensity of all claudins was found significantly lower in colorectal cancer tissues when compared to paired normal mucosa (p ≤ 0.001). For claudin 4, the percentage of cells staining positively was also significantly reduced (p = 0.04). In normal mucosa, cytoplasm showed no staining for claudins in any patient, whereas in paired colorectal cancer tissues, significant cytoplasmic staining appeared both for claudin 1 (p = 0.04) and claudin 4 (p = 0.01). Tumor samples were significantly hypomethylated in CLDN1 (p < 0.05). In conclusion, our results show that CLDN1 is significantly hypomethylated in tumor samples and that the membrane staining intensity for claudin 1, 4, and 7 is significantly lower in colorectal cancer tissues than in adjacent nonneoplastic tissue. Colorectal cancer cells showed dystopic cytoplasmic location of claudins.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
653		a Tight junction; methylation; colon cancer; claudin
653		a Biomedicin
653		a Biomedicine
700	1	a Askari, Shlearu Department of Clinical Research, Örebro University Hospital, Örebro, Sweden,Orebro Univ, Fac Med & Hlth, Dept Clin Res, Orebro, Sweden.4 aut
700	1	a Ahmad, Abrar,d 1984-u Department of Clinical Research, Örebro University Hospital, Örebro, Sweden,Orebro Univ, Fac Med & Hlth, Dept Clin Res, Orebro, Sweden.4 aut
700	1	a Befekadu, Rahel,d 1968-u Örebro universitet,Institutionen för medicinska vetenskaper,Department of Clinical Research, Örebro University Hospital, Örebro, Sweden,Orebro Univ, Fac Med & Hlth, Dept Clin Res, Orebro, Sweden.;Orebro Univ, Fac Med & Hlth, Dept Lab Med, Orebro, Sweden.4 aut0 (Swepub:oru)rlbu
700	1	a Nilsson, Torbjörn K.u Umeå universitet,Klinisk kemi,Umea Univ, Dept Med Biosci, Div Clin Chem, Umea, Sweden.4 aut0 (Swepub:umu)toni0049
710	2	a Uppsala universitetb Institutionen för medicinsk cellbiologi4 org
773	0	t Tumor Biologyd : SAGE Openg 39:4q 39:4x 1010-4283x 1423-0380
856	4	u https://doi.org/10.1177/1010428317697569y Fulltext
856	4	u https://journals.sagepub.com/doi/pdf/10.1177/1010428317697569
856	4	u https://umu.diva-portal.org/smash/get/diva2:1117532/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856	4	u https://uu.diva-portal.org/smash/get/diva2:1112585/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-57335
856	4 8	u https://doi.org/10.1177/1010428317697569
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-136210
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-323664

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Av författaren/redakt...: Hahn-Strömberg, ...; Askari, Shlear; Ahmad, Abrar, 19 ...; Befekadu, Rahel, ...; Nilsson, Torbjör ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Cancer och onkol ...

Artiklar i publikationen: Tumor Biology

Av lärosätet: Örebro universitet; Umeå universitet; Uppsala universitet

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