L773:1872 7913
Sökning: L773:1872 7913 > Detection of gyrA m...
- 33 av 96
- Föregående post
- Nästa post
- Till träfflistan
Detection of gyrA mutations associated with ciprofloxacin resistance in Neisseria gonorrhoeae by rapid and reliable pre-programmed short DNA sequencing
-
- Gharizadeh, Baback (författare)
- Stanford Univ, Stanford Genome Technol Ctr
-
- Akhras, Michael (författare)
- KTH,Skolan för bioteknologi (BIO)
-
- Unemo, Magnus (författare)
- Örebro Univ Hosp, Dept Clin Microbiol
- visa fler...
- visa färre...
- (creator_code:org_t)
- Elsevier BV, 2005
- 2005
- Engelska.
- Ingår i: International Journal of Antimicrobial Agents. - : Elsevier BV. - 0924-8579 .- 1872-7913. ; 26:6, s. 486-490
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Quinolone resistance is rapidly increasing in Neisseria gonorrhoeae and is posing a significant public health threat that requires constant surveillance. A rapid and reliable mutation detection assay has been developed. The assay is based on pre-programmed short DNA sequencing and is designed to detect point mutations in the gyrA gene that are highly related to ciprofloxacin resistance, i.e. in codons 91 and 95. By developing an assay based on pyrosequencing and exploiting the pre-programmed nucleotide dispensation capability of this technology, the sequence comprising the mutations will be analysed and promptly reveal whether the N. gonorrhoeae pathogen carries resistance to ciprofloxacin. A panel of 40 N. gonorrhoeae clinical isolates, of which 27 phenotypically displayed decreased susceptibility or resistance to ciprofloxacin, was used in the present study. All point mutations in the short stretch of the N. gonorrhoeae gyrA gene were easily discriminated, and the genotypic results obtained by pre-programmed sequencing were mainly in agreement with the phenotypically identified decreased susceptibility or resistance to ciprofloxacin. The new method used in the present study has the potential for rapid and reliable identification of known as well as previously unknown drug resistance mutations.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- DNA sequencing; ciprofloxacin resistance; Neisseria gonorrhoeae; pre-programmed DNA sequencing; pyrosequencing technology; ANTIMICROBIAL RESISTANCE; MECHANISMS; FAILURE; ISOLATE; PARC
- Biochemistry
- Biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
- International Journal of Antimicrobial Agents (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- 33 av 96
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Gharizadeh, Baba ...
- Akhras, Michael
- Unemo, Magnus
- Wretlind, Bengt
- Nyrén, Pål
- Pourmand, Nader
- Om ämnet
-
- NATURVETENSKAP
- NATURVETENSKAP
- och Biologi
- och Biokemi och mole ...
- Artiklar i publikationen
- International Jo ...
- Av lärosätet
- Kungliga Tekniska Högskolan
- Karolinska Institutet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
