The oxidative inactivation of plasminogen activator inhibitor type 1 results from a conformational change in the molecule and does not require the involvement of the P1' methionine.

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Fältnamn	Indikatorer	Metadata
000		02470naa a2200265 4500
001		oai:DiVA.org:umu-59899
003		SwePub
008		120926s1991 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-598992 URI
040		a (SwePub)umu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Strandberg, L4 aut
245	1 0	a The oxidative inactivation of plasminogen activator inhibitor type 1 results from a conformational change in the molecule and does not require the involvement of the P1' methionine.
264	1	c 1991
338		a print2 rdacarrier
520		a Plasminogen activator inhibitor 1 (PAI-1) is sensitive to oxidative inactivation, and it has been suggested that specific oxidation of a methionine residue, Met347, situated in the P1' position of the reactive center may be the cause of the inactivation. To test this hypothesis we have purified and biochemically characterized mutant proteins of PAI-1 in which Met347 and either of two other methionines, Met266 or Met354, has been replaced with oxidation-resistant valine residues. The mutant proteins were found to be equally sensitive to oxidation as wild-type PAI-1, suggesting that a specific oxidation of the P1' Met347 is not responsible for the inactivation. When PAI-1 was oxidized, circular dichroism analysis revealed a rapid conformational change that correlated to the loss of inhibitory activity. The oxidation sensitivity of PAI-1 was enhanced dramatically in the presence of 0.001% sodium dodecyl sulfate, and the circular dichroism spectrum was significantly different from that of untreated PAI-1, suggesting that the increased sensitivity to oxidation may be caused by a conformational change in the inhibitor molecule. Taken together, our data suggest that the oxidative inactivation of PAI-1 is not caused by the specific oxidation of the P1' methionine but results from a conformational change in the protein structure.
700	1	a Lawrence, D A4 aut
700	1	a Johansson, L B4 aut
700	1	a Ny, Toru Umeå universitet,Institutionen för medicinsk kemi och biofysik4 aut0 (Swepub:umu)tony0001
710	2	a Umeå universitetb Institutionen för medicinsk kemi och biofysik4 org
773	0	t Journal of Biological Chemistryg 266:21, s. 13852-8q 266:21<13852-8x 0021-9258x 1083-351X
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-59899

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Av författaren/redakt...: Strandberg, L; Lawrence, D A; Johansson, L B; Ny, Tor

Artiklar i publikationen: Journal of Biolo ...

Av lärosätet: Umeå universitet

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