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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005273naa a2200505 4500
001oai:DiVA.org:uu-233007
003SwePub
008140929s2014 | |||||||||||000 ||eng|
009oai:slubar.slu.se:65354
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2330072 URI
0247 a https://doi.org/10.1186/1756-3305-7-4102 DOI
0247 a https://res.slu.se/id/publ/653542 URI
040 a (SwePub)uud (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Tyden, Evau Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health4 aut0 (Swepub:slu)48108
2451 0a Deep amplicon sequencing of preselected isolates of Parascaris equorum in beta-tubulin codons associated with benzimidazole resistance in other nematodes
264 1b Springer Science and Business Media LLC,c 2014
338 a electronic2 rdacarrier
520 a Background: The development of anthelmintic resistance (AR) to macrocyclic lactones in the equine roundworm Parascaris equorum has resulted in benzimidazoles now being the most widely used substance to control Parascaris infections. However, over-reliance on one drug class is a risk factor for the development of AR. Consequently, benzimidazole resistance is widespread in several veterinary parasites, where it is associated with single nucleotide polymorphisms (SNPs) in drug targets encoded by the beta-tubulin genes. The importance of these SNPs varies between different parasitic nematodes, but it has been hypothesised that they occur, at low allele frequencies, even in unselected populations. This study investigated whether these SNPs exist in the P. equorum population and tested the hypothesis that BZ resistance can develop from pre-existing SNPs in codons 167, 198 and 200 of the beta-tubulin isotype 1 and 2 genes, reported to be associated with AR in strongylids. The efficacy of the oral paste formula fenbendazole on 11 farms in Sweden was also assessed. Methods: Two isotype-specific primer pairs were designed, one on either side of the codon 167 and one on either side of codons 198 and 200. A pool of 100 000 larvae was sequenced using deep amplicon sequencing by Illumina HiSeq. Faecal egg count reduction test was used to assess the efficacy of fenbendazole. Results: No SNPs were observed in codons 167, 198 or 200 of the beta-tubulin isotype 1 or 2 genes of P. equorum, even though 100 000 larvae were sequenced. Faecal egg count reduction testing of fenbendazole showed that this anthelmintic was still 100% effective, meaning that the likelihood of finding high allele frequency of SNPs associated with benzimidazoles resistance in P. equorum was low. Unexpectedly, the allele frequencies observed in single worms were comparable to those in pooled samples. Conclusions: We concluded that fenbendazole does not exert selection pressure on the beta-tubulin genes of isotypes 1 and 2 in P. equorum. The fact that no pre-existing SNPs were found in codons 167, 198 and 200 in P. equorum also illustrates the difficulties in generalising about AR mechanisms between different taxonomic groups of nematodes.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Medicinsk genetik0 (SwePub)301072 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medical Genetics0 (SwePub)301072 hsv//eng
650 7a LANTBRUKSVETENSKAPERx Veterinärmedicinx Patobiologi0 (SwePub)403022 hsv//swe
650 7a AGRICULTURAL SCIENCESx Veterinary Sciencex Pathobiology0 (SwePub)403022 hsv//eng
650 7a NATURVETENSKAPx Biologix Annan biologi0 (SwePub)106992 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Other Biological Topics0 (SwePub)106992 hsv//eng
653 a Parascaris equorum
653 a Anthelmintic resistance
653 a beta-tubulin
653 a SNP
653 a Fenbendazole
653 a Illumina HiSeq
7001 a Dahlberg, Johanu Uppsala universitet,Molekylär medicin,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)johda411
7001 a Karlberg, Olofu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär medicin4 aut0 (Swepub:uu)oka21721
7001 a Höglund, Johanu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health4 aut0 (Swepub:slu)47705
7102 a Sveriges lantbruksuniversitetb Inst för biomedicin och veterinär folkhälsovetenskap4 org
7102 a Sveriges lantbruksuniversitet
7730 t Parasites & Vectorsd : Springer Science and Business Media LLCg 7, s. 410-q 7<410-x 1756-3305
8564 u https://uu.diva-portal.org/smash/get/diva2:754192/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/1756-3305-7-410
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-233007
8564 8u https://doi.org/10.1186/1756-3305-7-410
8564 8u https://res.slu.se/id/publ/65354

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Tyden, Eva
Dahlberg, Johan
Karlberg, Olof
Höglund, Johan
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MEDICIN OCH HÄLSOVETENSKAP
MEDICIN OCH HÄLS ...
och Medicinska och f ...
och Medicinsk geneti ...
LANTBRUKSVETENSKAPER
LANTBRUKSVETENSK ...
och Veterinärmedicin
och Patobiologi
NATURVETENSKAP
NATURVETENSKAP
och Biologi
och Annan biologi
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Parasites & Vect ...
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Uppsala universitet
Sveriges Lantbruksuniversitet

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