Genome-wide identification of estrogen receptor alpha-binding sites in mouse liver

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Fältnamn	Indikatorer	Metadata
000		02885naa a2200301 4500
001		oai:prod.swepub.kib.ki.se:116389943
003		SwePub
008		240701s2008 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1163899432 URI
024	7	a https://doi.org/10.1210/me.2007-01212 DOI
040		a (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Gao, Hu Karolinska Institutet4 aut
245	1 0	a Genome-wide identification of estrogen receptor alpha-binding sites in mouse liver
264	1	b The Endocrine Society,c 2008
520		a We report the genome-wide identification of estrogen receptor α (ERα)-binding regions in mouse liver using a combination of chromatin immunoprecipitation and tiled microarrays that cover all nonrepetitive sequences in the mouse genome. This analysis identified 5568 ERα-binding regions. In agreement with what has previously been reported for human cell lines, many ERα-binding regions are located far away from transcription start sites; approximately 40% of ERα-binding regions are located within 10 kb of annotated transcription start sites. Almost 50% of ERα-binding regions overlap genes. The majority of ERα-binding regions lie in regions that are evolutionarily conserved between human and mouse. Motif-finding algorithms identified the estrogen response element, and variants thereof, together with binding sites for activator protein 1, basic-helix-loop-helix proteins, ETS proteins, and Forkhead proteins as the most common motifs present in identified ERα-binding regions. To correlate ERα binding to the promoter of specific genes, with changes in expression levels of the corresponding mRNAs, expression levels of selected mRNAs were assayed in livers 2, 4, and 6 h after treatment with ERα-selective agonist propyl pyrazole triol. Five of these eight selected genes, Shp, Stat3, Pdgds, Pck1, and Pdk4, all responded to propyl pyrazole triol after 4 h treatment. These results extend our previous studies using gene expression profiling to characterize estrogen signaling in mouse liver, by characterizing the first step in this signaling cascade, the binding of ERα to DNA in intact chromatin.
700	1	a Falt, Su Karolinska Institutet4 aut
700	1	a Sandelin, A4 aut
700	1	a Gustafsson, JAu Karolinska Institutet4 aut
700	1	a Dahlman-Wright, Ku Karolinska Institutet4 aut
710	2	a Karolinska Institutet4 org
773	0	t Molecular endocrinology (Baltimore, Md.)d : The Endocrine Societyg 22:1, s. 10-22q 22:1<10-22x 0888-8809x 1944-9917
856	4	u https://academic.oup.com/mend/article-pdf/22/1/10/8936765/mend0010.pdf
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:116389943
856	4 8	u https://doi.org/10.1210/me.2007-0121

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Av författaren/redakt...: Gao, H; Falt, S; Sandelin, A; Gustafsson, JA; Dahlman-Wright, ...

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