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Ischemic preconditi...
Ischemic preconditioning can overcome the effect of moderate to severe cold ischemia on concordant mouse xeno-heart transplants
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Lukes, D. J. (författare)
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Lundgren, A. (författare)
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Skogsberg, U. (författare)
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visa fler...
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Karlsson-Parra, Alex, 1950 (författare)
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- Soussi, Bassam, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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- Olausson, Michael, 1956 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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visa färre...
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(creator_code:org_t)
- 2005
- 2005
- Engelska.
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Ingår i: Transplantation proceedings. - 0041-1345. ; 37:8, s. 3332-4
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- PURPOSE: Concordant mouse xeno-heart transplants are relatively sensitive to ischemia-reperfusion injury. We investigated the effect of an ischemic preconditioning (IPC) protocol on the functional and biochemical outcome of mouse xenohearts transplanted to the Lewis rat. MATERIAL AND METHODS: NMRI mice (30 to 40 g) were anesthetized, intubated, and mechanically ventilated. They were subjected either to a IPC protocol leading to an SaO(2) of 70% for 5 minutes followed by normoxia (defined as SaO(2) >90%) for 10 minutes (n = 9) or normoxia only (n = 11). The hearts were then heterotopically transplanted to Lewis rats (220 g). The frequencies of immediate onset and early dysfunction and late dysfunction were registered. The hearts surviving for 6 hours were explanted and the absolute concentrations of phosphocreatine and adenosine triphosphate (ATP) were determined in micromole per gram of heart tissue with high-pressure liquid chromatography. The phosphorylation ratio, PCr/ATP, a known correlate to biochemical and functional outcome, was calculated. RESULTS: Four of 11 (36.4%) of control hearts experienced immediate onset and early dysfunction versus 0% (0/9) in M hearts subjected to IPC (P = .01). Furthermore, the IPC protocol increased the PCr concentration, 15.08 +/- 1.00 versus 9.04 +/- 2.04 micromol/g in controls (P = .01), and the PCr/ATP ratio, 1.80 +/- 0.17 versus 1.27 +/- 0.21 (NS; P = .06). CONCLUSIONS: IPC provides a protective PCr overshoot overcoming the short-term effects of moderate to severe ischemic injury on mouse xeno-heart transplants.
Nyckelord
- Animals
- Graft Survival
- Heart Transplantation/*physiology
- *Ischemic Preconditioning
- Mice
- Mice
- Inbred Strains
- Rats
- Rats
- Inbred Lew
- Transplantation
- Heterologous/*physiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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