Functional expression of mu-opioid receptors in the human colon cancer cell line, HT-29, and their localization in human colon.

Nylund, Gunnar, 1959 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences

Pettersson, Ann, 1982- (author): Högskolan i Kalmar,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences,Naturvetenskapliga institutionen,Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden

Bengtsson, Cecilia, 1980 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences

Khorram-Manesh, Amir, 1958 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences

Nordgren, Svante, 1945 (author): Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences

Delbro, Dick, 1950 (author): Högskolan i Kalmar,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences,Naturvetenskapliga institutionen,Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden

(creator_code:org_t)

2007-08-07
2008
English.
In: Digestive diseases and sciences. - : Springer Science and Business Media LLC. - 0163-2116 .- 1573-2568. ; 53:2, s. 461-6

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Abstract Subject headings

We have investigated the functional expression of mu-opioid receptors (MORs) in the human colon cancer cell line, HT-29. As revealed by immunocytochemistry, immunoreactivity was present in both the cytoplasm and nuclei of the cells. Challenge with morphine for 24 h (1 nM to 1 microM) barely affected cell proliferation, while the secretion of urokinase type plasminogen activator (a protease involved in invasion/metastasis) was markedly augmented by a concentration of 0.1 microM. Human colon cancer tissue from 14 consecutively operated patients was investigated by immunohistochemistry. MORs were found in the nuclei of colonocytes and immune cells of the lamina propria in tumor-free tissue. In tumor tissue, immunoreactivity was found in the membrane and often in the nuclei of tumor cells. The current findings suggest that morphine administration could affect tumor progression by interfering with, for example, invasive properties. Our demonstration of a nuclear expression of the MORs appears to be a novel finding.

By the author/editor: Nylund, Gunnar, ...; Pettersson, Ann, ...; Bengtsson, Cecil ...; Khorram-Manesh, ...; Nordgren, Svante ...; Delbro, Dick, 19 ...

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