Amyloid beta1-40 quantification in CSF: comparison between chromatographic and immunochemical methods.

• BACKGROUND/AIMS: Amyloid beta (Abeta) is the principal component of senile plaques, one of the hallmarks of Alzheimer's disease (AD). Evidence is accumulating that soluble aggregates (oligomers) of Abeta are important in the pathogenesis of AD. METHODS: We compared three different methods for quantification of the 40 amino acid form of Abeta (Abeta40) in CSF, two based on antibodies [ELISA and surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF) with antibody-coated arrays] and one based on direct binding of proteins to a protein array [SELDI-TOF and immobilized metal affinity [copper] (IMAC30)]. RESULTS: CSF Abeta40 concentration was only found to be significantly elevated in AD (127% of control levels; p=0.0095) using SELDI-TOF with IMAC30 arrays. CONCLUSIONS: These data suggest that the measured Abeta level in CSF may differ depending on whether antibody-based methods are used or not, possibly caused by epitope masking due to Abeta oligomerization or to binding of Abeta to carrier proteins.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Nyckelord

Aged

Aged

80 and over

Alzheimer Disease

cerebrospinal fluid

Amyloid beta-Protein

analysis

cerebrospinal fluid

Biological Markers

analysis

cerebrospinal fluid

Cerebrospinal Fluid

chemistry

Enzyme-Linked Immunosorbent Assay

methods

Female

Humans

Immunochemistry

methods

Male

Peptide Fragments

analysis

cerebrospinal fluid

Reference Values

Reproducibility of Results

Spectrometry

Mass

Matrix-Assisted Laser Desorption-Ionization

methods

Publikations- och innehållstyp

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art (ämneskategori)