Sökning: WFRF:(Thunberg S.) >
Epidermal Keratinoc...
Epidermal Keratinocyte Depletion during Five Weeks of Radiotherapy is Associated with DNA Double-Strand Break Foci, Cell Growth Arrest and Apoptosis: Evidence of Increasing Radioresponsiveness and Lack of Repopulation; The Number of Melanocytes Remains Unchanged
-
- Turesson, Ingela (författare)
- Uppsala universitet,Experimentell och klinisk onkologi
-
- Simonsson, M. (författare)
- Uppsala universitet,Experimentell och klinisk onkologi
-
- Hermansson, I. (författare)
- Univ Göteborg, Sahlgrenska Univ Hosp, Dept Oncol, Gothenburg, Sweden.
-
visa fler...
-
- Book, M. (författare)
- Uppsala Univ, Dept Immunol Genet & Pathol, Expt & Clin Oncol, Uppsala, Sweden.
-
- Sigurdadottir, S. (författare)
- Uppsala universitet,Experimentell och klinisk onkologi
-
- Thunberg, Ulf (författare)
- Uppsala universitet,Experimentell och klinisk onkologi
-
- Qvarnström, F. (författare)
- Uppsala universitet,Experimentell och klinisk onkologi
-
- Johansson, Karl-Axel (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för radiofysik,Institute of Clinical Sciences, Department of Radiation Physics,Univ Göteborg, Sahlgrenska Univ Hosp, Dept Radiophys, Gothenburg, Sweden.
-
- Fessé, Per (författare)
- Uppsala universitet,Centrum för klinisk forskning, Gävleborg
-
- Nyman, J. (författare)
- Univ Göteborg, Sahlgrenska Univ Hosp, Dept Oncol, Gothenburg, Sweden.
-
visa färre...
-
(creator_code:org_t)
- 2020
- 2020
- Engelska.
-
Ingår i: Radiation Research. - 0033-7587 .- 1938-5404. ; 193:5, s. 481-496
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- During fractionated radiotherapy, epithelial cell populations are thought to decrease initially, followed by accelerated repopulation to compensate cell loss. However, previous findings in skin with daily 1.1 Gy dose fractions indicate continued and increasing cell depletion. Here we investigated epidermal keratinocyte response with daily 2 Gy fractions as well as accelerated and hypofractionation. Epidermal interfollicular melanocytes were also assessed. Skin-punch biopsies were collected from breast cancer patients before, during and after mastectomy radiotherapy to the thoracic wall with daily 2 Gy fractions for 5 weeks. In addition, 2.4 Gy radiotherapy four times per week and 4 Gy fractions twice per week for 5 weeks, and two times 2 Gy daily for 2.5 weeks, were used. Basal keratinocyte density of the interfollicular epidermis was determined and immunostainings of keratinocytes for DNA double-strand break (DSB) foci, growth arrest, apoptosis and mitosis were quantified. In addition, interfollicular melanocytes were counted. Initially minimal keratinocyte loss was observed followed by pronounced depletion during the second half of treatment and full recovery at 2 weeks post treatment. DSB foci per cell peaked towards the end of treatment. p21-stained cell counts increased during radiotherapy, especially the second half. Apoptotic frequency was low throughout radiotherapy but increased at treatment end. Mitotic cell count was significantly suppressed throughout radiotherapy and did not recover during weekend treatment gaps, but increased more than threefold compared to unexposed skin 2 weeks post-radiotherapy. The number of melanocytes remained constant over the study period. Germinal keratinocyte loss rate increased gradually during daily 2 Gy fractions for 5 weeks, and similarly for hypofractionation. DSB foci number after 2 Gy irradiation revealed an initial radioresistance followed by increasing radiosensitivity. Growth arrest mediated by p21 strongly suggests that cells within or recruited into the cell cycle during treatment are at high risk of loss and do not contribute significantly to repopulation. It is possible that quiescent (G0) cells at treatment completion accounted for the accelerated post-treatment repopulation. Recent knowledge of epidermal tissue regeneration and cell cycle progression during genotoxic and mitogen stress allows for a credible explanation of the current finding. Melanocytes were radioresistant regarding cell depletion. © 2020 by Radiation Research Society. All rights of reproduction in any form reserved.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Nyckelord
- protein p21
- adjuvant radiotherapy
- apoptosis
- Article
- breast cancer
- cancer adjuvant therapy
- cancer patient
- cancer radiotherapy
- cell count
- cell cycle progression
- cell growth
- clinical article
- double stranded DNA break
- growth disorder
- human
- human tissue
- hypofractionated radiotherapy
- immunohistochemistry
- interfollicular epidermis
- keratinocyte
- mastectomy
- melanocyte
- mitosis
- priority journal
- punch biopsy
- radiosensitivity
- skin biopsy
- thorax wall
- tissue regeneration
- treatment duration
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Turesson, Ingela
-
Simonsson, M.
-
Hermansson, I.
-
Book, M.
-
Sigurdadottir, S ...
-
Thunberg, Ulf
-
visa fler...
-
Qvarnström, F.
-
Johansson, Karl- ...
-
Fessé, Per
-
Nyman, J.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Cancer och onkol ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Radiologi och bi ...
- Artiklar i publikationen
-
Radiation Resear ...
- Av lärosätet
-
Göteborgs universitet
-
Uppsala universitet