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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03819naa a2200661 4500 | |
| 001 | oai:gup.ub.gu.se/282314 | |
| 003 | SwePub | |
| 008 | 240528s2019 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://gup.ub.gu.se/publication/2823142 URI |
| 024 | 7 | a https://doi.org/10.1016/j.jmoldx.2019.03.0062 DOI |
| 040 | a (SwePub)gu | |
| 041 | a eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a He, H. J.4 aut |
| 245 | 1 0 | a Multilaboratory Assessment of a New Reference Material for Quality Assurance of Cell-Free Tumor DNA Measurements |
| 264 | 1 | b Elsevier BV,c 2019 |
| 520 | a We conducted a multilaboratory assessment to determine the suitability of a new commercially available reference material with 40 cancer variants in a background of wild-type DNA at four different variant allele frequencies (VAFs): 2%, 0.50%, 0.125%, and 0%. The variants include single nucleotides, insertions, deletions, and two structural variations selected for their clinical importance and to challenge the performance of next-generation sequencing (NGS) methods. Fragmented DNA was formulated to simulate the size distribution of circulating wild-type and tumor DNA in a synthetic plasma matrix. DNA was extracted from these samples and characterized with different methods and multiple laboratories. The various extraction methods had differences in yield, perhaps because of differences in chemistry. Digital PCR assays were used to measure VAFs to compare results from different NGS methods. Comparable VAFs were observed across the different NGS methods. This multilaboratory assessment demonstrates that the new reference material is an appropriate tool to determine the analytical parameters of different measurement methods and to ensure their quality assurance. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
| 653 | a liquid biopsies | |
| 653 | a quantification | |
| 653 | a accuracy | |
| 653 | a rare | |
| 653 | a bias | |
| 653 | a kits | |
| 653 | a Pathology | |
| 700 | 1 | a Stein, E. V.4 aut |
| 700 | 1 | a Konigshofer, Y.4 aut |
| 700 | 1 | a Forbes, T.4 aut |
| 700 | 1 | a Tomson, F. L.4 aut |
| 700 | 1 | a Garlick, R.4 aut |
| 700 | 1 | a Yamada, E.4 aut |
| 700 | 1 | a Godfrey, T.4 aut |
| 700 | 1 | a Abe, T.4 aut |
| 700 | 1 | a Tamura, K.4 aut |
| 700 | 1 | a Borges, M.4 aut |
| 700 | 1 | a Goggins, M.4 aut |
| 700 | 1 | a Elmore, S.4 aut |
| 700 | 1 | a Gulley, M. L.4 aut |
| 700 | 1 | a Larson, J. L.4 aut |
| 700 | 1 | a Ringel, L.4 aut |
| 700 | 1 | a Haynes, B. C.4 aut |
| 700 | 1 | a Karlovich, C.4 aut |
| 700 | 1 | a Williams, P. M.4 aut |
| 700 | 1 | a Garnett, A.4 aut |
| 700 | 1 | a Ståhlberg, Anders,d 1975u Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för biomedicin,Institute of Biomedicine4 aut0 (Swepub:gu)xsandw |
| 700 | 1 | a Filges, Stefan,d 1991u Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center,Institutionen för biomedicin,Institute of Biomedicine4 aut0 (Swepub:gu)xfilst |
| 700 | 1 | a Sorbara, L.4 aut |
| 700 | 1 | a Young, M. R.4 aut |
| 700 | 1 | a Srivastava, S.4 aut |
| 700 | 1 | a Cole, K. D.4 aut |
| 710 | 2 | a Göteborgs universitetb Sahlgrenska Cancer Center4 org |
| 773 | 0 | t Journal of Molecular Diagnosticsd : Elsevier BVg 21:4, s. 658-676q 21:4<658-676x 1525-1578 |
| 856 | 4 | u http://www.jmdjournal.org/article/S1525157818304793/pdf |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/282314 |
| 856 | 4 8 | u https://doi.org/10.1016/j.jmoldx.2019.03.006 |
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